PKD通过ERK1/2信号通路调控单核细胞增生李斯特菌感染Caco-2细胞  

PKD regulates Listeria monocytogenes infection in Caco-2 cells via the ERK1/2 signaling pathway

作　　者：李家政 张峰源 许晶晶 姜旭淦 陈盛霞 LI Jia-zheng;ZHANG Feng-yuan;XU Jing-jing;JIANG Xu-gan;CHEN Sheng-xia(Department of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang 212013,China;Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Suzhou 215000,China)

机构地区：[1]江苏大学医学院检验医学系,镇江212013 [2]南京中医药大学附属苏州市中医医院,苏州215000

出　　处：《中国人兽共患病学报》2023年第10期927-932,共6页Chinese Journal of Zoonoses

基　　金：科技部基础平台项目(No.2019-194-30)。

摘　　要：目的探讨蛋白激酶D(PKD)在单核细胞增生李斯特菌(Lm)感染Caco-2细胞中的作用及其机制。方法建立Lm感染Caco-2细胞模型,qRT-PCR检测PKD1、PKD2和PKD3 mRNA表达水平。加入PKD抑制剂CID755673后,Lm感染Caco-2细胞,CCK-8法测定细胞存活率,Western blot法检测细胞炎症相关蛋白和ERK1/2信号通路蛋白的表达。结果Lm感染促进Caco-2细胞表达PKD;与Lm组相比,抑制PKD可明显降低Caco-2细胞存活率(F=4.831,P<0.05),明显抑制炎症相关蛋白NLRP3、p-IκBα(F_(NLRP3)=35.02,F_(p-IκBα)=45.19,均P<0.01)和ERK1/2蛋白(F=326.9,P<0.001)的表达。结论PKD-ERK1/2信号通路在Lm感染Caco-2细胞中发挥重要调控作用。This study was aimed at investigating the role and mechanism of protein kinase D(PKD)in the infection of Caco-2 cells by Listeria monocytogenes(Lm).The mRNA expression levels of PKD1,PKD2,and PKD3 were determined by qRT-PCR in Caco-2 cells infected with Lm.After the addition of the PKD inhibitor CID755673,Caco-2 cells were infected with Lm;cell viability was determined with CCK-8 assays;and the expression of inflammation related proteins and ERK1/2 signaling pathway proteins was determined by western blotting.The expression of PKD was elevated in Caco-2 cells infected with Lm.Compared with the Lm group,the group with inhibition of PKD showed significantly lower Caco-2 cell viability(F=4.831,P<0.05),as well as significant inhibition of the expression of the inflammation related proteins NLRP3 and p-IκBα(F_(NLRP3)=35.02,F_(p-IκBα)=45.19,all P<0.01)and the proteins ERK1/2(F=326.9,P<0.001).These results indicated that the PKD-ERK1/2 signaling pathway played an important regulatory role in Caco-2 cells infected with Lm.

关 键 词：单核细胞增生李斯特菌 CACO-2 蛋白激酶D ERK1/2信号通路 炎症 

分 类 号：R378.994[医药卫生—病原生物学]