基于新型拼接光子晶体光纤的SPR葡萄糖浓度传感器  

SPR Glucose Concentration Sensor Based on Novel Cascaded Photonic Crystal Fiber

作　　者：谭启龙 冯国英[1] 李筱薇 谭建昌 韩敬华[1] 石中兵[2] TAN Qilong;FENG Guoying;LI Xiaowei;TAN Jianchang;HAN Jinghua;SHI Zhongbing(Inst.of Laser Micro-Nano Eng.,College of Electronics and Info.Eng.,Sichuan Univ.,Chengdu 610065,China;Southwestern Inst.of Physics,Chengdu 610041,China)

机构地区：[1]四川大学电子信息学院激光微纳工程研究所,四川成都610065 [2]核工业西南物理研究院,四川成都610041

出　　处：《工程科学与技术》2023年第6期46-53,共8页Advanced Engineering Sciences

基　　金：等离子体物理重点实验室基金项目(6142A04200210);国家自然科学基金委员会–中国工程物理研究院联合基金项目(U1730141);国家重点研发计划项目(2017YFE0301203)。

摘　　要：针对人体血糖的稳定高效检测问题,本文提出了一种基于表面等离子体共振(SPR)技术的光纤传感器并成功应用于葡萄糖浓度检测。采用光纤熔接机级联拼接多模光纤–光子晶体光纤–光子晶体光纤–多模光纤(MPPM),利用磁控溅射仪在光纤结构表面沉积金膜。随后,使用巯基乙胺对传感区的金膜进行修饰,采用戊二醛固定葡萄糖氧化酶(GOD)的技术路线,使传感器具备葡萄糖特异性识别功能,并实现对低浓度葡萄糖溶液的传感。针对SPR传感器的折射率灵敏度,本文设计两组对比实验,分别是相同结构不同传感区长度的传感器对比,以及相同长度不同结构的传感器对比。研究结果表明:传感区长度对传感器的折射率灵敏度的影响较小;MPPM传感结构的折射率灵敏度优于MPM传感结构(提高了约101.84 nm/RIU),且MPPM结构的传感器具有更深的SPR共振峰,意味着检测精度更高。对于制备的葡萄糖传感器,设计了一套葡萄糖浓度、特异性识别及稳定性的检测方案。实验结果表明:在0~0.8 mg/mL的浓度范围的葡萄糖溶液中,MPPM传感器的葡萄糖灵敏度约为29.61nm/(mg·mL^(–1)),且表现出良好的线性关系,拟合系数达到了0.954;具有良好的特异性识别能力及稳定性能。相比于传统的葡萄糖传感器,本文提出的葡萄糖传感器结构紧凑、生物相容性好、灵敏度高。Aiming at the problem of stable and efficient detection of human blood glucose,an optical fiber sensor based on surface plasmon resonance(SPR)technology was proposed and successfully applied to the detection of glucose concentration in this paper.The structure of MMF(multimode fiber)–PCF(photonic crystal fiber)–PCF–MMF(MPPM)was spliced using the fiber fusion machine,then the gold film was deposited on its surface by magnetron sputtering.The gold film in the sensing region was modified with mercaptoethylamine,and glucose oxidase(GOD)was immobilized by glutaraldehyde to make the sensor with glucose specific recognition and low concentration glucose sensing was realized.For the refractive index sensitivity of the SPR sensor,the comparison experiments of two groups of sensors were designed.One was the comparison of sensors with the same structure and different sensing area lengths,and the other was the comparison of sensors with the same length and different structures.The research results showed that the length of the sensing region has a few effect on the refractive index sensitivity of the sensor;and the refractive index sensitivity of the MPPM structure is better than that of the MPM structure(improved about 101.84 nm/RIU),and the sensor of the MPPM structure has a deeper SPR resonance peak,which means higher detection accuracy.For the glucose sensor,a set of detection schemes for glucose concentration,specific recognition and stability were designed.It showed good linearity in the glucose concentration range of 0~0.8 mg/mL,with the glucose sensitivity of 29.61 nm/(mg/ml)and the fitting coefficient of 0.954.Compared with the traditional glucose sensor,the glucose sensor proposed in the paper has a compact structure,superior biocompatibility and high sensitivity.

关 键 词：表面等离子共振 光子晶体光纤 光纤传感 葡萄糖浓度传感 

分 类 号：TN249[电子电信—物理电子学]