荔枝多胺氧化酶(PAO)基因家族的全基因组鉴定与表达分析  

Genome-wide Identification and Expression Analysis of Polyamine Oxidase(PAO)Gene in Litchi chinensis

作　　者：唐丽雯 张月 岳晓琦 丁峰 胡福初[4] 周开兵[1,2] 马武强 Tang Liwen;Zhang Yue;Yue Xiaoqi;Ding Feng;Hu Fuchu;Zhou Kaibing;Ma Wuqiang(Sanya Nanfan Research Institute of Hainan University,Sanya,572025;Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province,Horticulture College,Hainan University,Haikou,570311;Guangxi Crop Genetic Improvement and Biotechnology Key Laboratory,Guangxi Academy of Agricultural Sciences,Nanning,530007;Investigation Station of Tropical Fruit Trees(Ministry of Agriculture),Key Laboratory of Tropical Fruit Tree Biology of Hainan Province,Tropical Fruit Trees Institute,Hainan Academy of Agricultural Sciences,Haikou,571100)

机构地区：[1]海南大学三亚南繁研究院,三亚572025 [2]海南大学园艺学院,海南省热带园艺作物品质调控重点实验室,海口570228 [3]广西农业科学院,广西作物遗传改良生物技术重点开放实验室,南宁530007 [4]海南省农业科学院热带果树研究所,海南省热带果树生物学重点实验室,农业部海口热带果树科学观测实验站,海口571100

出　　处：《分子植物育种》2023年第22期7360-7371,共12页Molecular Plant Breeding

基　　金：海南省基础与应用基础研究计划(自然科学领域)青年项目(320QN191)、海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC082);海南省重大科技计划项目(ZDKJ2021006);海南大学引进高层次人才启动基金[KYQD(2R)1970];广西自然科学基金项目(2022GXNSFDA080010)共同资助。

摘　　要：为了探究PAO基因在荔枝(Litchi chinensis Sonn.)生长发育过程中的生物学功能,本研究基于荔枝全基因组数据,利用生物信息学方法筛选与鉴定荔枝PAO基因(LcPAO)成员,并对其理化性质、系统发育、结构、染色体定位、顺式作用元件、蛋白结构、miRNA靶位点和表达模式进行了分析。结果表明,一共鉴定得到10个LcPAO基因,全部为亲水蛋白。系统发育分析发现LcPAO落在4个亚族中的Ⅰ、Ⅱ和Ⅲ亚族。LcPAO1~LcPAO3和LcPAO9~LcPAO10属于典型的PAO蛋白,LcPAO4~LcPAO8还含有一个特异的SWIRM结构域,属于非典型PAO蛋白。顺式作用元件分析表明其主要含光响应元件、逆境胁迫相关元件以及激素响应相关元件。miRNA靶位点分析发现LcPAO基因受到22个miRNAs的靶向调控。转录组表达模式分析发现,除了LcPAO1和LcPAO4,余下的LcPAO基因可能与荔枝果皮着色相关。所有的LcPAO基因均可能与荔枝的化学控花相关。除了LcPAO1所有的LcPAO基因均可能与荔枝果实脱落相关。本研究为深入研究LcPAO基因的功能和调控机制提供参考。In order to explore the biological function of polyamine oxidase(PAO)gene in the growth and development of Litchi chinensis,based on the whole genome data of Litchi,the PAO gene members of Litchi chinensis(LcPAO)were screened and identified,and their physicochemical properties,structure,phylogeny,chromosomal localization,cis-acting elements,protein structure,miRNA target site and expression patterns were analyzed by bioinformatics.The results showed that a total of 10 LcPAO genes were identified and all of them were hydrophilic proteins.Phylogenetic analysis exhibited that LcPAO genes fell intoⅠ,ⅡandⅢsubgroups of the four subgroups.LcPAO1~LcPAO3 and Lc PAO9~LcPAO10 belonged to typical PAO proteins,and LcPAO4~LcPAO8belonged to atypical PAO proteins which contained a specific SWIRM domain.Cis-acting elements analysis displayed that them mainly contained light response elements,stress related elements and hormone response related elements.miRNA target site analysis found that LcPAO genes were targeted by 22 miRNAs.Transcriptome expression pattern analysis demonstrated that in addition to LcPAO1 and LcPAO4,the remaining LcPAO genes may be related to Litchi chinensis pericarp coloring.All LcPAO genes may be involved in the chemical flower control of Litchi.All LcPAO genes except LcPAO1 may be related to Litchi fruit abscission.This study provides a reference for further study on the function and regulation mechanism of LcPAO genes.

关 键 词：荔枝 多胺氧化酶(PAO) 生物信息学 表达分析 

分 类 号：S667.1[农业科学—果树学]