巴西橡胶树乳管细胞中与HbMago2相互作用蛋白的筛选与分析  

Screening and Analysis of Proteins Interacting with HbMago2 in Laticifer Cells of Hevea brasiliensis

作　　者：杨子平[1,2,3] 郭冬[1,4,3] 李辉亮 朱家红[1,4,3] 王颖 彭世清[1,4] Yang Ziping;Guo Dong;Li Huiliang;Zhu Jiahong;Wang Ying;Peng Shiqing(Key Laboratory of Biology and Genetic Resources of Tropical Crops(Ministry of Agriculture and Rural Affairs),Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101;Zhanjiang Key Laboratory of Tropical Crop Genetic Improvement,South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101;Sanya Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,524091;Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province,Hainan Institute for Tropical Agricultural Resources,Sanya,572025)

机构地区：[1]中国热带农业科学院热带生物技术研究所,农业农村部热带作物生物技术重点开放实验室,海口571101 [2]中国热带农业科学院南亚热带作物研究所,湛江市热带作物遗传改良重点实验室,海口571101 [3]中国热带农业科学院三亚研究院,湛江524091 [4]海南热带农业资源研究院,海南省热带农业生物资源保护与利用重点实验室,三亚572025

出　　处：《分子植物育种》2023年第22期7392-7398,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31970620,32171827);中央级公益性科研院所基本科研业务费专项(1630052022-009)共同资助。

摘　　要：为探索HbMago2在乳管细胞中的作用,本研究构建了HbMago2的诱饵载体,从巴西橡胶树乳管cDNA表达文库中筛选与HbMago2相互作用的蛋白。结果显示,诱饵载体无自激活性、对酵母菌Y2HGold无毒性;通过文库筛选,获得3个与HbMago2相互作用的蛋白;点对点验证结果表明HbMago2能够与Y14、phox和HMAD在酵母中能相互作用。互作蛋白中phox是烟酰胺腺嘌呤二核苷酸磷酸氧化酶(NADPH)的亚基,NADPH氧化酶能引起ROS,而重金属相关结构域蛋白(HMAD)能清除重金属引起的ROS,推测HbMago2通过蛋白互作协调乳管细胞中ROS的平衡。本研究结果为进一步研究HbMago2在乳管细胞中的功能提供重要的研究基础。In order to explore the functions of HbMago2 in laticifer cells,a bait vector of HbMago2 was constructed for yeast two-hybrid,and proteins that interacted with HbMago2 were screened from a latex cDNA expression library of Hevea brasiliensis in this study.The results showed that the bait vector had no autoactivation and was non-toxic to the yeast strain Y2HGold.Through library screening,we obtained three proteins from a yeast two-hybrid library of laticifer cells c DNA using yeast two-hybrid system.One-to-one testing results showed that HbMago2 could interact with Y14,phox and HMAD in yeast.The phox is a subunit of nicotinamide adenine dinucleotide phosphate oxidase(NADPH)in interacting proteins.NADPH oxidase enzyme could cause ROS,and heavy-metal-associated domain-containing protein(HMAD)could clear ROS caused by heavy metals.We inferred that HbMago2 coordinates the balance of ROS in laticifer cells through protein-protein interactions.The results of this study provide an important research foundation for further investigating the function of HbMago2 in laticifer cells.

关 键 词：橡胶树 Mago Nashi 酵母双杂交 蛋白相互作用 

分 类 号：S794.1[农业科学—林木遗传育种]