健脾清化方对非酒精性脂肪性肝病模型小鼠细胞凋亡的影响  

Study on effects of Jianpi Qinghua Decoction on hepatocyte apoptosis in nonalcoholic fatty liver disease model mice

作　　者：韩煦 蔡梦洁 陈清光[1] 田静 陆灏[1] Han Xu;Cai Mengjie;Chen Qingguang;Tian Jing;Lu Hao(Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院内分泌科,上海201203

出　　处：《国际中医中药杂志》2023年第11期1382-1385,共4页International Journal of Traditional Chinese Medicine

基　　金：上海市重点专科-中医内分泌项目(shslczdzk05401);上海中医药大学附属曙光医院四明基金项目(SGKG-202012)。

摘　　要：目的研究健脾清化方对非酒精性脂肪性肝病(NAFLD)模型小鼠肝细胞凋亡的影响,探讨其作用机制。方法选择29只C57小鼠予60%高脂饲料喂养16周,制备NAFLD模型,另设6只小鼠为正常组,予普通饲料喂养。造模成功后,选择体重较大的12只小鼠按随机数字表法分为中药组和模型组,同时继续喂养高脂饲料。中药组灌胃健脾清化方20.961 g/kg,正常组和模型组灌胃等体积的蒸馏水,1次/d,连续干预4周。采用ELISA法检测血清GOT、GPT水平,油红O染色检测肝组织脂质沉积情况,TUNEL荧光染色检测肝细胞凋亡情况,采用Western blot检测肝组织cleaved Caspase-3、Caspase-3、Bax、Bcl-2及JNK、p-JNK蛋白表达。结果与模型组比较,中药组小鼠体重降低(P<0.05),血清GPT水平降低(P<0.05),肝组织脂质沉积及凋亡细胞减少,肝组织cleaved Caspase-3/Caspase-3比值、p-JNK/JNK比值及Bax蛋白表达降低(P<0.05),Bcl-2蛋白表达升高(P<0.05)。结论健脾清化方可抑制JNK蛋白磷酸化,减少NAFLD小鼠肝细胞凋亡,从而可能延缓NAFLD向肝硬化和肝癌的发展进程。Objective To study the effects of Jianpi Qinghua Decoction on hepatocyte apoptosis under non-alcoholic fatty liver disease(NAFLD),and to discuss its mechanism.Methods Totally 29 C57 mice were randomly selected and fed a 60%high fat diet for 16 weeks,while the remaining 6 mice were given regular feed as the normal group.After successful modeling,12 mice with larger body weight were divided into TCM group and model group using a random number table method,while continuing to receive high-fat feed.The TCM group was orally administered Jianpi Qinghua Decoction 20.961 g/kg.The normal group and model group were orally administered an equal volume of distilled water once a day,with continuous intervention for 4 weeks.The levels of GOT and GPT in blood were detected by ELISA,the deposition of triglyceride in liver was detected by oil red O,and the apoptosis of liver cells was detected by TUNEL fluorescence staining.Western blot was used to detect the expressions of cleaved Caspase-3,Caspase-3,Bax,Bcl-2,JNK and p-JNK.Results Compared with the model group,the body weight and GPT in the TCM group significantly decreased(P<0.05),TG deposition was significantly reduced,apoptosis range of liver cells was significantly reduced,and cleaved Caspase-3/Caspase-3,p-JNK/JNK and the expression of Bax significantly decreased(P<0.05).The expression of Bcl-2 increased(P<0.05).Conclusion Jianpi Qinghua Decoction can inhibit JNK protein phosphorylation and effectively reduce liver cell apoptosis in NAFLD mice,which may delay the progression of NAFLD towards cirrhosis and liver cancer.

关 键 词：非酒精性脂肪肝 健脾清化方 肝细胞 细胞凋亡 小鼠 

分 类 号：R285.5[医药卫生—中药学]