基于免疫抑制指标优化芍藤方提取工艺研究  

Study on optimization of extraction process of Shaoteng Decoction based on immunosuppressive index

作　　者：庞枫韬 李克嵩 唐晓颇[1] Pang Fengtao;Li Kesong;Tang Xiaopo(Department of Rheumatology,Guang'anmen Hospital,Chinese Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]中国中医科学院广安门医院风湿病科,北京100053

出　　处：《国际中医中药杂志》2023年第11期1386-1390,共5页International Journal of Traditional Chinese Medicine

基　　金：中央级公益性科研院所任务项目(ZZ15-XY-PT-11);中国中医科学院科技创新工程项目(CI2021A01502)。

摘　　要：目的通过对3种不同提取方式获得的芍藤方配方颗粒进行药效学研究,探索芍藤方的免疫抑制作用,优选提取工艺。方法分别采用水提取、70%乙醇提取及水提醇沉工艺制备芍藤方提取物。将30只非肥胖型糖尿病(NOD)小鼠按随机数字表法分为芍藤方全方水提组(工艺一组)、芍藤方70%乙醇提取组(工艺二组)、芍藤方水提醇沉组(工艺三组)、模型组和羟氯喹组,每组6只。另设6只Balb/C小鼠为正常对照组。工艺一、二、三组灌胃相应芍藤方提取物16 g/kg,羟氯喹组灌胃硫酸羟氯喹60 mg/kg,正常对照组和模型组灌胃等体积去离子水,1次/d,连续灌胃4周。末次给药后取材,计算脾脏指数和颌下腺指数,采用HE染色观察颌下腺组织病理形态,采用ELISA法测定血清IL-1β、IL-6、TNF-α、脱氢表雄酮(DHEA)、雌二醇和IgG水平。结果与模型组比较,各给药组脾脏指数降低(P<0.01或P<0.05),工艺三组和羟氯喹组颌下腺指数升高(P<0.01);各给药组颌下腺组织病理评分降低(P<0.01或P<0.05),血清IL-1β、IL-6、TNF-α、IgG水平降低(P<0.01或P<0.05),DHEA水平升高(P<0.01或P<0.05);工艺一组、工艺三组和羟氯喹组血清雌二醇水平升高(P<0.01或P<0.05)。结论芍藤方可有效降低NOD小鼠血清炎症因子水平并抑制免疫,以芍藤方水提醇沉工艺的提取物药效更优,为其最佳提取工艺。Objective To study the pharmacodynamics of Shaoteng Decoction obtained by three different extraction methods;To explore the immunosuppressive effect of Shaoteng Decoction;To optimize the extraction process.Methods The extract of Shaoteng Decoction was prepared by water extraction,70%ethanol extraction and water extraction and alcohol precipitation respectively.Totally 30 NOD mice were randomly divided into Shaoteng Decoction water extraction group(processⅠ),Shaoteng Decoction 70%ethanol extraction group(processⅡ),Shaoteng Decoction water extraction and alcohol precipitation group(processⅢ),model group and hydroxychloroquine group according to their body weight,with 6 mice in each group.Another 6 Balb/C mice were set as normal control group.In the processⅠ,processⅡ,and processⅢgroups,16 g/kg of Shaoteng Decoction extract was given by gavage,while in the hydroxychloroquine group,60 mg/kg of hydroxychloroquine sulfate was given by gavage.The normal control group and the model group were given the same volume of deionized water once a day for 4 weeks.After the last administration,the spleen index and submandibular gland index were calculated,the pathological morphology of submandibular gland was observed by HE staining,and the serum levels of IL-1β,IL-6,TNF-α,dehydroepiandrosterone(DHEA),estradiol(E2)and immunoglobulin G(IgG)were determined by ELISA.Results Compared with the model group,the spleen index of each drug group decreased(P<0.01 or P<0.05),and the submandibular gland index of the processⅢgroup and hydroxychloroquine group increased(P<0.01).The pathological score of submandibular gland in each administration group decreased(P<0.01 or P<0.05),the serum levels of IL-1β,IL-6,TNF-αand IgG decreased(P<0.01 or P<0.05),and the DHEA level increased(P<0.01 or P<0.05).The level of serum E2 in process groupⅠ,process groupⅢand hydroxychloroquine group increased(P<0.01 or P<0.05).Conclusion Shaoteng Decoction can effectively reduce inflammation and inhibit immunity of NOD mice.In addition,the extrac

关 键 词：干燥综合征 芍藤方 炎症 免疫抑制 提取法 小鼠 近交NOD 

分 类 号：R284.2[医药卫生—中药学]