高效液相色谱⁃三重四极杆质谱法同时测定藏药制剂吉尼德协中12种成分的含量  

Simultaneous determination of 12 components in Ji⁃Ni De⁃Xie by HPLC⁃QqQ⁃MS

作　　者：彭芳 赵紫薇 罗玉婷 易欢 伍金丹增 范刚[1,2] PENG Fang;ZHAO Zi-wei;LUO Yu-ting;YI Huan;WU Jin-dan-zeng;FAN Gang(College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Dege Tibetan Institute of Tibetan Hospital,Dege 627250,China)

机构地区：[1]成都中医药大学药学院,成都611137 [2]成都中医药大学民族医药学院,成都611137 [3]德格县藏医院(藏医药研究所),德格627250

出　　处：《中国新药杂志》2023年第19期2001-2008,共8页Chinese Journal of New Drugs

基　　金：四川省重点研发项目(2022YFS0434)。

摘　　要：目的:建立同时测定吉尼德协中小檗碱、木兰花碱、姜黄素等12种成分含量的方法。方法:以WondaSil C18⁃WR色谱柱,流动相为0.1%甲酸(A)⁃甲醇(B)进行梯度洗脱;采用高效液相色谱⁃三重四极杆质谱(HPLC⁃QqQ⁃MS)仪,电喷雾离子源(ESI)温度为300℃,带电离子在正、负离子模式同时扫描,多重反应监测模式下检测;依据12种对照品线性方程计算5批样品中12种成分的含量。结果:吉尼德协中12种成分在检测范围内线性关系良好。5个批次吉尼德协中木兰花碱、药根碱、小檗碱、姜黄素、去甲氧基姜黄素、没食子酸、诃黎勒酸、阿魏酸4⁃O⁃β⁃D⁃吡喃葡萄糖苷、羟基红花黄色素A、芦丁、阿魏酸、鞣花酸的平均含量分别为3.18,0.20,1.71,0.52,0.22,15.93,7.56,3.29,0.07,0.20,1.47,2.89 mg·g^(-1)。结论:本研究所建立的分析方法专属性强、灵敏度高,可为吉尼德协的质量控制提供参考。Objective:To establish the method of simultaneous determination of 12 major chemical components(such as berberine,magnolflorine and curcumin)in Tibetan medicine Ji⁃Ni De⁃Xie.Methods:The separation was achieved on a WondaSil C18⁃WR column.High performance liquid chromatography⁃triple quadrupole mass spectrometry(HPLC⁃QqQ⁃MS)was used with the mobile phase 0.1%formic acid solution(A)⁃methanol(B)under gradient elution.The temperature of Electron Spray Ionization(ESI)was 300℃.The charged ions were scanned in positive and negative ion modes at the same time with multiple reaction monitoring.The contents of 12 components in 5 batches of samples were calculated according to the linear equation of 12 reference substances.Results:The 12 components of the Ji⁃Ni De⁃Xie have a good linear relationship within the detection range.The average contents of magnolflorine,jatrorrhizine,berberine,curcumin,demethoxycurcumin,gallic acid,chebulagic acid,ferulic acid 4⁃O⁃β⁃D⁃glucopyranoside,hydroxysafflor yellow A,rutin,ferulic acid,and ellagic acid are 3.18,0.20,1.71,0.52,0.22,15.93,7.56,3.29,0.07,0.20,1.47,2.89 mg·g^(-1),respectively.Conclusion:The established analytical method is highly selective with high sensitivity.providing reference for the quality control of the Tibetan medicine Ji⁃Ni De⁃Xie.

关 键 词：藏药 吉尼德协 高效液相色谱⁃三重四极杆质谱 含量测定 

分 类 号：R917[医药卫生—药物分析学]