外源CO供体高铁血红素对盐胁迫下加工番茄苗期根系形态及生理特性的影响  

Effects of exogenous hemin of exogenous CO on root morphology and physiological characteristics of processed tomato seedlings under salt stress

作　　者：滕元旭 骆霞 张雪蒙 崔辉梅[1,2] TENG Yuan-xu;LUO Xia;ZHANG Xue-meng;CUI Hui-mei(Agricultural College,Shihezi University,Shihezi,Xinjiang 832003,China;Xinjing Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization,Shihezi,Xinjiang 832003,China)

机构地区：[1]石河子大学农学院,新疆石河子832003 [2]特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子832003

出　　处：《西南农业学报》2023年第9期1906-1914,共9页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31860551)。

摘　　要：【目的】研究盐胁迫下喷施不同浓度CO供体对加工番茄幼苗的影响,为探究CO供体高铁血红素在提高番茄耐盐性等领域提供理论基础。【方法】以加工番茄品系KT⁃32为试验材料,采用水培法,设置霍格兰营养液培养(CK)和盐胁迫(100 mmol/L NaCl)+喷施4种浓度的外源CO供体(0、0.05、0.10、0.15、0.25μmol/L)处理,测定各处理幼苗根系形态、渗透调节物质含量、活性氧含量、保护酶活性、膜脂过氧化程度及细胞膜透性,结合方差分析与主成分分析,分析在100 mmol/L NaCl胁迫下最适外源CO供体浓度。【结果】盐胁迫会使加工番茄幼苗的根系生长受到抑制,使总根长、根体积、根系平均直径下降,叶片中活性氧含量增多,叶绿素和蛋白质降解,细胞膜结构受到伤害,激活保护酶系统。当外源施加CO供体时盐胁迫对加工番茄的伤害得到缓解,在15μmol/L(H_(3))时效果最佳,在盐处理9 d时,与NaCl处理相比,总根长、根投影面积、根表面积、根系平均直径、根体积分别提高24.22%、38.16%、38.16%、24.51%、53.97%,叶绿素a、叶绿素b、总叶绿素含量分别提高29.04%、34.73%、30.75%,渗透性调节物质脯氨酸(Pro)含量、可溶性糖含量和叶片相对含水分别提高40.68%、55.20%和117.34%,叶片中活性氧(ROS)超氧阴离子(O_(2)^(-·))产生速率和过氧化氢(H_(2)O_(2))含量分别降低47.34%和20.91%,保护酶超氧化物歧化酶(SOD)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)活性分别提高36.19%、97.23%和135.35%,丙二醛(MDA)含量、相对电导率分别降低57.82%、19.35%;对加工番茄幼苗根系形态及生理等21个指标经归一化处理后进行主成分分析显示H3处理的综合得分高于其他盐胁迫处理,在所有处理中排第2,为2.25。【结论】外源CO供体高铁血红素通过提高盐胁迫下加工番茄幼苗的抗氧化酶活性和渗透调节物质,降低膜脂过氧化程度提高植株光合色素含量,维持�【Objective】The paper aimed to study the effects of spraying different concentrations of CO donors on processed tomato seedlings under salt stress,and provided a theoretical basis for exploring the role of CO in areas such as improving salt tolerance in tomato.【Method】Processed tomato line KT⁃32 was used as the experimental material,and four treatments of exogenous CO donors(0,0.05,0.10,0.15,0.25 mmol/L)were set up by hydroponic culture with Hoagland nutrient solution culture(CK)and salt stress(100 mmol/L NaCl)+spraying,the root morphology,osmoregulatory substance content,reactive oxygen species(ROS),membrane lipid activity degree and cell membrane permeability of each treatment were measured.Combining analysis of variance and principal component analysis,the optimal con⁃centration of exogenous CO donors under 100 mmol/L NaCl stress was analyzed.【Result】Salt stress inhibited root growth of processed tomato seedlings,causing a decrease in total root length,root volume,and average root diameter,an increase in reactive oxygen species content in leaves,degradation of chlorophyll and proteins,and damaged to cell membrane structure and activation of protective enzyme systems.The injury of salt stress on processed tomatoes was alleviated when CO donors were exogenously applied,with the best effect at a concentration of 15μmol/L(H_(3)),and at 9 days of salt treatment,total root length,root projected area,root surface area,root mean diameter,and root vol⁃ume were increased by 24.22%,38.16%,38.16%,24.51%,24.51%,and 53.97%,chlorophyll a,chlorophyll b,and total chloro⁃phyll content increased by 29.04%,34.73%,and 30.75%,respectively,and osmoregulatory substance proline content,soluble sugar content,and relative water content of leaves increased by 40.68%,55.20%,and 117.34%,respectively,and the rate of reactive oxygen species(ROS)O_(2)^(-·) production and H_(2)O_(2) content in leaves decreased by 47.34%and 20.91%,respectively;The activities of protective enzymes SOD,POD and APX increased by 36.19%,97.23%an

关 键 词：一氧化碳 加工番茄 盐胁迫 根系形态 生理指标 

分 类 号：S641.2[农业科学—蔬菜学]