挤压综合征大鼠核心脏器肾心肺损伤特点研究  

Characteristics of injury in kidney, heart, and lung of rats with crush syndrome

作　　者：张妍[1,2] 吕强 韩笑 王旭 刘冉[1] 张利 陈香美[1] Zhang Yan;Lyu Qiang;Han Xiao;Wang Xu;Liu Ran;Zhang Li;Chen Xiangmei(Deparment of Nephrology,First Medical Center of Chinese PLA General Hospital,Chinese PLA Institute of Nephrology,State Key Laboratory of Kidney Diseases,National Clinical Research Center for Kidney Diseases,Beijing Key Laboratory of Kidney Diseases;Chinese PLA Medical School,Beijing 100853,China)

机构地区：[1]解放军总医院第一医学中心肾脏病医学部、解放军肾脏病研究所、肾脏疾病国家重点实验室、国家慢性肾病临床医学研究中心、肾脏疾病研究北京市重点实验室,北京100853 [2]解放军医学院,北京100853

出　　处：《中华肾病研究电子杂志》2023年第5期248-253,共6页Chinese Journal of Kidney Disease Investigation（Electronic Edition）

基　　金：国家重点研发计划(2021YFC3002203)。

摘　　要：目的:构建挤压综合征(CS)模型,初步阐明核心脏器肾心肺的损伤特点及功能改变。方法:16只雄性SD大鼠随机分为对照组(Sham组)和挤压综合征组(CS组)。CS组采用数字化动物挤压模拟平台在3 kg压力下持续挤压双后肢12 h,构建挤压综合征模型。24 h后,所有动物检测血清肌酸激酶、肌酐、尿素氮、肌钙蛋白等生化指标变化;支气管肺泡灌洗液细胞数、蛋白浓度及髓过氧化物酶(MPO)活性变化;超声心动图观察心脏功能变化;PAS、HE染色观察组织形态学改变。结果:与Sham组相比,CS组大鼠血清肌酸激酶、肌酐和尿素氮等升高(P均<0.05),支气管肺泡灌洗液细胞数增多(P=0.032),蛋白浓度增加及MPO活性增强(P=0.015);心脏超声显示心舒张功能降低(P<0.05);组织病理提示:肾脏小管上皮细胞脱落、小管扩张、蛋白管型形成,肺间质细胞数明显增多。结论:大鼠挤压伤24 h后,核心脏器肾心肺均出现明显功能障碍,肾、肺病理可见明显损伤。Objective To establish a rat model of crush syndrome(CS)and preliminarily clarify the injury characteristics and functional changes in kidney,heart,and lung.Methods Sixteen male SD rats were randomly divided into the sham group and CS group with 8 rats each.In the CS group,the hind limbs of rats were crushed under a pressure of 3 kg for 12 h by means of a digital animal crush injury simulation platform to establish the CS model.Twenty-four hours after successful model establishment,the rats were measured in the levels of serum creatine kinase,creatinine,urea nitrogen,and troponin,etc.The bronchoalveolar lavage fluid were detected for the number of cells,protein concentration,and myeloperoxidase(MPO)activity.Echocardiography was performed to detect the changes of cardiac function.Staining methods of PAS and HE were used to observe the histopathological changes in kidney,lung,and heart of the rats.Results Compared with the sham group,the CS group showed higher levels of serum creatine kinase,creatinine,and urea nitrogen(all P<0.05).The CS group also displayed significant increase in cell numbers(P=0.032),protein concentration,and MPO activity(P=0.015)in the bronchoalveolar lavage fluid.And the CS group diclosed obvious cardiac diastolic dysfunction as well(P<0.05).Besides,histopathological observations found detachment of renal tubular epithelial cells,expansion of renal tubules,renal tubular cast,and increase in the number of pulmonary interstitial cells.Conclusion Twenty-four hours after the crush injury,the rats showed significant dysfunction in the kidneys,heart,and lung,and the pathological damage to the kidney and lung was obvious.

关 键 词：挤压综合征 损伤 肾脏 肺脏 心脏 

分 类 号：R692[医药卫生—泌尿科学]