猪链球菌2型、7型及9型小鼠感染模型的初步建立  

Development of an Experimental Mouse Model for Streptococcus suis Type 2,7,9 Infection

作　　者：闫祖杰 潘如意 张俊杰 马晓春 魏建超[2] 李蓓蓓[2] 刘珂[2] 邱亚峰[2] 马志永[2] 刘聚祥[1] YAN Zujie;PAN Ruyi;ZHANG Junjie;MA Xiaochun;WEI Jianchao;LI Beibei;LIU Ke;QIU Yafeng;MA Zhiyong;LIU Juxiang(College of Veterinary Medicine,HeBei Agricultural University,Baoding 071000,China;Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China)

机构地区：[1]河北农业大学动物医学院,保定071000 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2023年第4期150-157,共8页Chinese Journal of Animal Infectious Diseases

基　　金：中国科学技术交流中心(0301001999302)。

摘　　要：本实验为了研究猪链球菌2型(SS2)、猪链球菌7型(SS7)及猪链球菌9型(SS9)菌株对BALB/c小鼠的致病性,探究BALB/c小鼠作为猪链球菌动物感染模型的可行性以及作为后续实验的依据的可靠性。本研究通过腹腔注射猪链球菌感染BALB/c小鼠,对死亡小鼠进行无菌解剖分离菌株并培养,对培养的菌株进行革兰氏染色法和PCR鉴定;同时利用荧光定量的方法检测各个脏器细菌的载量和组织切片HE染色观察脏器的病理变化。实验结果显示:攻毒的小鼠出现典型的临床症状并死亡,经过实验验证后确定小鼠是由于感染猪链球菌而致病、致死;测定SS2、SS7及SS9的LD_(50)分别为3.7×10^(7)、4.1×10^(7)、7.9×10^(7) CFU/mL。因此BALB/c小鼠对猪链球菌易感,可以作为实验室研究猪链球菌良好的实验动物。In order to study the pathogenicity of Streptococcus suis type 2 (SS2),S.suis type 7 (SS7) and S.suis type 9 (SS9) strains,an experimental animal model was developed in BALB/c mice in the present study.BALB/c mice were intraperitoneally injected with S.suis cultures.The dead mice were aseptically dissected to isolate the bacteria.Then,the cultured bacteria were identified by Gram staining and PCR.At the same time,real-time quantitative PCR was used to detect the bacterial loads of viscera and HE staining of tissue sections to observe the pathological changes.The experimental results showed that the mice inoculated with the S.suis developed typical clinical signs and died.These deaths were confirmed due to S.suis infection.The LD_(50) doses for mouse death were 3.7×10^(7),4.1×10^(7) and7.9×10^(7) CFU/mL for SS2,SS7 and SS9,respectively.Therefore,BALB/c mice were susceptible to S.suis and might be used as good laboratory animals for the study of S.suis.

关 键 词：猪链球菌 BALB/C小鼠 动物模型 荧光定量 

分 类 号：S858.28[农业科学—临床兽医学]