尿液中14种挥发性有机物测定的自动顶空气相色谱-质谱法  

Determination of 14 volatile organic compounds in urine by headspace gas chromatography-mass spectrometry

作　　者：郑三燕[1] 方美璇 马奇发 李小东[1] 陈珞洛[1] Zheng Sanyan;Fang Meixuan;Ma Qifa;Li Xiaodong;Chen Luoluo(Physical and Chemical Institute of Wenzhou Center for Disease Control and Prevention,Wenzhou 325000,China)

机构地区：[1]温州市疾病预防控制中心理化所,温州325000

出　　处：《中华劳动卫生职业病杂志》2023年第10期850-854,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：温州市基础性公益科研项目(Y20210385)。

摘　　要：目的:建立自动顶空气相色谱-质谱联用法测定尿液中14种常见挥发性有机物的分析方法。方法:于2022年9月,取10 ml尿样于20 ml顶空瓶,65 ℃条件下平衡30 min,采用顶空技术和气相色谱-质谱应用技术检测,外标法定量。结果:14种挥发性有机物在0.2~8.0 μg/L和0.1~4.0 μg/L内线性关系良好,相关系数范围为0.995 6~0.999 9,添加3个不同浓度水平,回收率79.8%~113.1%,相对标准偏差0.05%~0.27%,检出限0.03~0.05 μg/L。结论:该检测方法操作简单、方便,回收率与精密度均符合要求,可运用于尿中常见挥发性有机物的检测。Objective To establish a automatic headspace gas chromatography-mass spectrometry(HS/GC-MS)method for the determination of 14 volatile organic compounds in urine.Methods In September 2022,10 ml urine sample was taken into a 20 ml headspace bottle,balanced for 30 min at 65℃,and then detected by HS/GC-MS and quantified by external standard method.Results The 14 volatile organic compounds showed good linearity at 0.2-8.0μg/L and 0.1-4.0μg/L,with correlation coefficients ranging from 0.9956-0.9999.The recoveries were 79.8%-113.1%with relative standard deviations 0.05%-0.27%when three different concentration levels were added.Detection limit was 0.03-0.05μg/L.Conclusion The method is simple and convenient,and the recovery and precision meet the requirements.It can be used for the determination of common volatile organic compounds in urine.

关 键 词：气相色谱-质谱法 有机物 尿 

分 类 号：O657.63[理学—分析化学] R446.12[理学—化学]