工作场所空气中4-甲基-2-戊醇测定的溶剂解吸-气相色谱法  

Determination of 4-methyl-2-pentanol in workplace air by solvent desorption-gas chromatography

作　　者：陈冠林 谭强 冯耀基[2] 兰红军[2] 杨学望 周雪琼 Chen Guanlin;Tan Qiang;Feng Yaoji;Lan Hongjun;Yang Xuewang;Zhou Xueqiong(Laboratory Department,Foshan Institute of Occupational Disease Prevention and Control,Foshan Occupational Health Research Institute,Foshan 528000,China;Laboratory Department,Foshan Nanhai District Center for Disease Control and Prevention,Foshan 528200,China;Department of Occupational Health and Occupational Medicine,School of Public Health,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]佛山市职业病防治所,佛山市职业健康研究院检验科,佛山528000 [2]佛山市南海区疾病预防控制中心检验科,佛山528200 [3]南方医科大学公共卫生学院职业卫生与职业医学系,广州510515

出　　处：《中华劳动卫生职业病杂志》2023年第10期859-862,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：广东省基础与应用基础研究基金(2021A1515010007);广东省医学科学技术研究基金项目(A2015617、A2018340、B2021212);佛山市医学类科技攻关项目(1920001000268);佛山市医学科研项目(20200399);南方医科大学科研启动计划项目(QD2018N007);国家自然科学基金青年科学基金(81903350);广东省职业卫生骨干培训项目(粤卫办函(2015)422号)。

摘　　要：目的:建立工作场所空气中4-甲基-2-戊醇的气相色谱测定法。方法:于2022年1月,以活性炭管采集工作场所空气中的4-甲基-2-戊醇,用二氯甲烷-甲醇(95∶5,V/V)解吸,经毛细管色谱柱分离后,气相色谱法测定。结果:4-甲基-2-戊醇的检出限为0.04μg/ml。4-甲基-2-戊醇的线性范围为0.16~1616.60μg/ml,回归方程为y=1.94 x-5.48,相关系数为0.99958,最低检出浓度为0.03 mg/m^(3)(空气体积以1.50 L计算),批内精密度为1.08%~1.75%,批间精密度为1.41%~2.52%,解吸效率为95.15%~99.91%。样品在室温下至少可以保存3 d,在4℃冰箱中至少可保存7 d。结论:该测定方法精密度好、灵敏度高、操作简单,适用于工作场所空气中4-甲基-2-戊醇的测定。Objective To establish a method for the determination of 4-methyl-2-pentanol in the air of workplace by gas chromatography.Methods In January 2022,4-methyl-2-pentanol in the air of workplace was collected by activated carbontube,eluted with dichloromethane-methanol(95∶5,V/V),separated by capillary column and determined by gas chromatogram.Results The limit of detection for 4-methyl-2-pentanol was 0.04μg/ml.The linear range of 4-methyl-2-pentanol was 0.16-1616.60μg/ml,with the regression equation of y=1.94x-5.48,and the coefficient correlation was 0.99958,and the minimum detection concentration was 0.03 mg/m^(3)(collected sample volume was 1.50 L).The within-run precisions were 1.08%-1.75%and the between-run precisions were 1.41%-2.52%.The desorption rates were 95.15%-99.91%.The samples could be stored at least 3 days at room temperature and 7 days at 4℃without significant loss.Conclusion The method has the advantages of good precision,high sensitivity and simple operation.It is suitable for the determination of 4-methyl-2-pentanol in the air of workplace.

关 键 词：色谱法 气相 4-甲基-2-戊醇 工作场所 空气 溶剂解吸 

分 类 号：R134[医药卫生—劳动卫生]