广东省两个地区2021-2022年百日咳鲍特菌分离株的抗菌药物敏感性和抗原基因型  

Antimicrobial susceptibility and antigen genotypes of Bordetella pertussis strains isolated in two prefectures of Guangdong province in 2021-2022

作　　者：李振翠 马炜森 王安娜[5] 张昌 余青紫 马伟峰 刘美真[1,2,3] Li Zhencui;Ma Weisen;Wang Anna;Zhang Chang;Yu Qingzi;Ma Weifeng;Liu Meizhen(Institute of Pathogenic Microbiology,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510000,Guangdong,China;Guangdong Key Laboratory for Research and Application of Emerging Infectious Disease Emergency Detection Technology,Guangzhou 510000,Guangdong,China;Guangdong Workstation for Emerging Infectious Disease Control and Prevention,Guangzhou 510000,Guangdong,China;Department of Microbiology,School of Public Health,Southern Medical University,Guangzhou 510515,Guangdong,China;Microbiology Laboratory,Guangzhou Center for Disease Control and Prevention,Guangzhou 510440,Guangdong,China)

机构地区：[1]广东省疾病预防控制中心病原微生物检验所,广东广州510000 [2]广东省新发传染病应急检测技术研究与应用重点实验室,广东广州510000 [3]广东新发突发传染病防治工作站,广东广州510000 [4]南方医科大学公共卫生学院微生物学系,广东广州510515 [5]广州市疾病预防控制中心微生物学检验部,广东广州510440

出　　处：《中国疫苗和免疫》2023年第5期498-502,共5页Chinese Journal of Vaccines and Immunization

基　　金：广东省医学科学技术研究基金(A2021461)。

摘　　要：目的了解广东省两个地区百日咳鲍特菌(Bordetella pertussis,Bp)抗菌药物敏感性和抗原基因型。方法通过MALDI-TOF-MS微生物质谱、荧光定量PCR和血清凝集试验对广州市和佛山市2021-2022年Bp分离株进行鉴定,采用E-test法进行10种抗菌药物的敏感性检测,利用二代测序平台和生物信息学分析软件进行全基因组测序和拼接,采用BLASTn软件分析抗原基因型和23 S rRNA基因突变。结果在15株Bp分离株中,12株(80.0%)对大环内酯类抗菌药物(红霉素、阿奇霉素和克拉霉素)耐药,且与Bp疫苗株相比存在23 S rRNA A2047 G突变,所有菌株对磺胺类、β-内酰胺类和喹诺酮类抗菌药物敏感;13株和2株分离株的抗原基因型分别为prn2/ptxA1/ptxC2/ptxP3/fim2-1/fim3-1/tcfA2和prn1/ptxA1/ptxC1/ptxP1/fim2-1/fim3-1/tcfA2。结论研究地区Bp分离株对大环内酯类抗菌药物的耐药率高,抗原基因型与疫苗株存在差异;需持续加强Bp流行株抗原基因型和药物敏感性监测。Objective To explore the antimicrobial susceptibility and antigen genotypes of Bordetella pertussis(Bp) strains isolated in two prefectures of Guangdong province.Methods We identified Bp strains in Guangzhou and Foshan cities from 2021 to 2022 using the MALDI-TOF-MS method,real-time PCR,and the serum agglutination test.We tested for susceptibility to 10 antibacterial drugs using the E-test method.Whole genomes of Bp strains were sequenced and assembled using the next generation sequencing platform and bioinformatics analysis software.We searched for 23 S rRNA gene mutations and antigen genotypes using the BLASTn software.Results Twelve(80.0%) of 15 Bp strains were resistant to macrolide antibacterial drugs(erythromycin,azithromycin,and clarithromycin) and had 23 S rRNA A2047 G mutations compared with the Bp vaccine strain.All Bp isolates were susceptible to sulfonamide,β-lactam,and quinolone antibacterials.Antigen genotypes for 13 Bp isolates were prn2/ptxA1/ptxC2/ptxP3/fim2-1/fim3-1/tcfA2 and for 2 strains were prn1/ptxA1/ptxC1/ptxP1/fim2-1/fim3-1/tcfA2.Conclusions Bp isolates in the study areas had high resistance to macrolide antibacterial drugs and antigen genotypes different from the vaccine strain.We should continue monitoring Bp antigen genotypes and antimicrobial susceptibility.

关 键 词：百日咳 百日咳鲍特菌 抗菌药物敏感性 抗原基因型 

分 类 号：R186[医药卫生—流行病学] R515.9[医药卫生—公共卫生与预防医学]