湖北省荆门市2022年蚊虫流行性乙型脑炎病毒携带调查  

Carriage of Japanese encephalitis virus in mosquitoes in Jingmen city of Hubei province,2022

作　　者：彭延[1] 蔡昆[1] 李静[1] 徐军强[1] 张婷[1] 熊进峰[1] 邹文菁[1] Peng Yan;Cai Kun;Li Jing;Xu Junqiang;Zhang Ting;Xiong Jinfeng;Zou Wenjing(Hubei Province Center for Disease Prevention and Control,Wuhan 430079,Hubei,China)

机构地区：[1]湖北省疾病预防控制中心,湖北武汉430079

出　　处：《中国疫苗和免疫》2023年第5期544-547,共4页Chinese Journal of Vaccines and Immunization

摘　　要：目的了解湖北省荆门市蚊虫流行性乙型脑炎(乙脑)病毒(Japanese encephalitis virus,JEV)携带状况。方法2022年7-8月在荆门市3个镇/街道选取7个点采集蚊虫标本,采用形态学方法鉴定蚊虫种类,采用实时荧光定量聚合酶链反应检测JEV核酸,计算蚊种的批阳性率和最低现场感染率,采用逆转录聚合酶链反应对JEV的E基因进行扩增和序列分析。结果共采集4920只蚊虫标本,其中致倦库蚊、白蚊伊蚊、骚扰阿蚊、中华按蚊分别占56.10%、41.46%、1.83%、0.61%;仅2批次致倦库蚊的JEV核酸检测阳性,批阳性率、最低现场感染率分别为6.67%(2/30)、0.07%(2/2760)。2株JEV分离株均为基因Ⅰ型,与中国浙江省和陕西省分离株亲缘关系最近。结论调查地区2022年蚊虫标本JEV携带率较低,蚊虫中存在基因Ⅰ型JEV循环。需持续开展蚊种JEV监测,警惕乙脑流行风险。Objective To explore carriage of Japanese encephalitis virus(JEV)in mosquitoes in Jingmen city of Hubei province.Methods We collected mosquito samples at 7 sites of 3 townships in Jingmen from July to August 2022 and identified mosquito types based on morphology.We used real-time polymerase chain reaction(PCR)to test for JEV nucleic acid to determine lot positivity rates and minimum infection rates(MIR)of mosquito types.We used reverse transcription PCR to amplify E genes of JEV strains for sequence analyses.Results A total of 4920 mosquito samples were collected,of which Culex quinquefasciatus,Aedes albopictus,Armigeres molestus,and Anopheles sinensis accounted for 56.10%,41.46%,1.83%,and 0.61%of samples,respectively.Only 2 lots of Culex quinquefasciatus were positive to JEV nucleic acid,for a lot-positivity rate of 6.67%(2/30)and an MIR of 0.07%(2/2760).The two JEV strains belonged to genotypeⅠand had close phylogenetic association with isolates from Zhejiang and Shaanxi provinces of China.Conclusions The carriage level of JEV in mosquitoes was low in 2022 in the surveyed area,with genotype I JEV circulating.We should continue JEV surveillance in mosquitoes and be alert to the risk of JE epidemics.

关 键 词：流行性乙型脑炎病毒 携带 基因特征 蚊虫 

分 类 号：R373.3[医药卫生—病原生物学]