UPLC法分析比较栀子和水栀子中主要成分的含量  

Determination and comparison of contents of main components in Gardenia Fructus and Gardenia Grandiflora Fructus by UPLC

作　　者：蔡晓洋 刘聪[1] 李敏[1] CAI Xiao-yang;LIU Cong;LI Min(School of Pharmacy,State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan)

机构地区：[1]成都中医药大学药学院西南特色中药资源国家重点实验室,四川成都611137

出　　处：《中药与临床》2023年第5期7-10,共4页Pharmacy and Clinics of Chinese Materia Medica

基　　金：四川省科技计划项目(No.18ZYF0970)。

摘　　要：目的:分析测定并比较栀子和水栀子中主要成分(栀子苷、西红花苷-1和西红花苷-2)的含量。方法:采用Waters ACQUITY UPLC BEH C18色谱柱(2.1 mmX50 mm,1.7μm),流动相乙腈-水,梯度洗脱,检测波长240,440nm,流速0.3 mL·min^(-1),柱温30℃,进样量2μL。结果:栀子苷在10.1~161.6μg·mL^(-1)内呈良好线性(r^(2)=0.9996),西红花苷-1在2.04~32.64μg·mL^(-1)内呈良好线性(r^(2)=0.9998),西红花苷-2在0.65625~10.5μg·mL^(-1)内呈良好线性(r^(2)=0.9996);各成分的平均回收率均大于95%。水栀子在栀子苷含量和西红花苷-1、西红花苷-2总含量上均高于栀子。结论:该方法简便、快速、准确、重复性好,可用于桅子和水栀子中主要成分的检测;水栀子在药用价值上具有良好的开发前景。Objective:To determine and compare the main constituent(geniposide,crocin-1 and crocin-2) contents of Gardenia Fructus and Gardenia Grandiflora Fructus by UPLC.Method:The samples were separated at 30 ℃ on a Waters ACQUITY UPLC BEH C18 column( 2.1 mm×50 mm,1.7 μm) and eluted with acetonitrile and water in gradient.The flow rate was 0.3 mL·min^(-1) and the detection wavelength were set at 240 nm and 440 nm.The injection volume was 2 μL.Result:The calibration curve of geniposide was linear within the range from 10.1 to 161.6 μg·mL^(-1)(r^(2)=0.9996).The calibration curve of crocin-1 was linear within the range from 2.04 to 32.64 μg·mL^(-1)(r^(2)=0.9998).The calibration curve of crocin-2 was linear within the range from 0.656 25 to 10.5 μg·mL^(-1)(r^(2)=0.9996).The average recoveries were all over 95%.the total content of geniposide and crocin-1,crocin-2 in Gardeniae Grandiflora Fructus were higher than that in Gardeniae Fructus.Conclusion:The method is simple,rapid,accurate,reproducible and used in the analysis of main components in Gardeniae Fructus and Gardeniae Grandiflora Fructus.Gardeniae Grandiflora Fructus has a good prospect of development as medicine.

关 键 词：栀子 水栀子 UPLC 栀子苷 西红花苷-1 西红花苷-2 

分 类 号：R282[医药卫生—中药学]