Effect of mono-/divalent metal ions on the conductivity characteristics of DNA solutions transferring through a microfluidic channel  

作　　者：朱洁 薛菁 赵伟 张琛 冯晓强 王凯歌 Jie Zhu;Jing Xue;Wei Zhao;Chen Zhang;Xiaoqiang Feng;Kaige Wang(State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials,Key Laboratory of Optoelectronic Technology of Shaanxi Province,National Center for International Research of Photoelectric Technology&Nano-functional Materials and Application,Institute of Photonics and Photon-Technology,Northwest University,Xi’an 710069,China)

机构地区：[1]State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials,Key Laboratory of Optoelectronic Technology of Shaanxi Province,National Center for International Research of Photoelectric Technology&Nano-functional Materials and Application,Institute of Photonics and Photon-Technology,Northwest University,Xi’an 710069,China

出　　处：《Chinese Physics B》2023年第11期161-167,共7页中国物理B（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (Grant Nos. 62275216 and 61775181);the Innovation Capability Support Program of Shaanxi Province of China (Grant Nos. S2018-ZC-TD-0061 and TZ0393);the National Key Scientific Instrument and Equipment Development Projects of China (Grant No. 51927804)。

摘　　要：Interactions between deoxyribonucleic acid(DNA) and metal ions are vital for maintaining life functions, however,there are still unsolved questions about its mechanisms. It is of great practical significance to study these issues for medical chip design, drug development, health care, etc. In this investigation, the conductivity properties of λ-DNA solutions with mono-/divalent metal ions(Na+, K^(+), Mg^(2+), and Ca^(2+)) are experimentally studied as they are electrically driven through a 5 μm microfluidic channel. Experimental data indicate that the conductivities of λ-DNA solutions with metal ions(M+/M2+) basically tend to reduce firstly and then increase as the voltage increases, of which the turning points varied with the metal ions. When the voltage surpasses turning points, the conductivity of λ-DNA-M+solutions increases with the concentration of metal ions, while that of λ-DNA-M^(2+)solutions decrease. Moreover, the conductivity of λ-DNA-M^(2+)solutions is always smaller than that of λ-DNA-M+solutions, and with high-concentration M^(2+), it is even smaller than that of the λ-DNA solution. The main reasons for the above findings could be attributed to the polarization of electrodes and different mechanisms of interactions between metal ions and λ-DNA molecules. This investigation is helpful for the precise manipulation of single DNA molecules in micro-/nanofluidic space and the design of new biomedical micro-/nanofluidic sensors.

关 键 词：MICROFLUIDICS interaction between deoxyribonucleic acid(DNA)and metal ions conductivity-voltage relations asymmetric electrodes 

分 类 号：R318[医药卫生—生物医学工程]