捏脊疗法对功能性消化不良大鼠胃窦平滑肌L-型钙通道及胃动力的影响  

Effects of chiropractic therapy on L-type calcium channels in gastric antral smooth muscles and gastric motility in rats with functional dyspepsia

作　　者：陈英英[1,2] 吴秋君 李华南 张玮[1,2] 包安[1,2] 刘书芹 王金贵[1,2] CHEN Yingying;WU Qiujun;LI Huanan;ZHANG Wei;BAO An;LIU Shuqin;WANG Jingui(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300193,China;National Administration of Traditional Chinese Medicine Massage Biological Effect of LevelⅢLaboratory,Tianjin 300193,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学第一附属医院,国家中医针灸临床医学研究中心,天津300193 [2]国家中医药管理局推拿手法生物效应三级实验室,天津300193 [3]天津中医药大学,天津301617

出　　处：《中华中医药杂志》2023年第11期5431-5435,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：区域中医(推拿)诊疗中心项目(No.20200424);天津市科技计划项目(No.21JCQNJC01200);国家自然科学基金项目(No.82205308);国家中医药管理局中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者(No.国中医药人教函[2021]203号);天津市教委科研计划项目(No.2017KJ148)。

摘　　要：目的:研究捏脊疗法对功能性消化不良(FD)大鼠胃窦平滑肌L-型电压依赖性钙离子通道(L-型钙通道)和Ca^(2+)浓度以及胃排空率、小肠推进率的影响。方法:用随机数表法将大鼠分为空白组、模型组、干预组,每组11只。采用不规则喂养、0℃0.9%氯化钠溶液灌胃联合夹尾刺激构建FD模型。干预组每日进行捏脊治疗,模型组每日进行相同条件的束缚,但不捏脊,空白组不做干预。检测各组大鼠胃排空率、小肠推进率、胃窦平滑肌Ca^(2+)浓度、L-型钙通道Cacnalc亚基mRNA和蛋白的表达。结果:捏脊治疗后,与空白组比较,模型组大鼠胃排空率、小肠推进率均显著下降(P<0.05,P<0.01),胃窦平滑肌Ca^(2+)浓度显著升高(P<0.01),胃窦平滑肌L-型钙通道Cacnalc亚基mRNA和蛋白水平显著升高(P<0.05,P<0.01);与模型组比较,干预组大鼠胃排空率、小肠推进率均显著上升(P<0.05),胃窦平滑肌Ca2+浓度显著下降(P<0.01),胃窦平滑肌L-型钙通道Cacnalc亚基蛋白水平显著下降(P<0.01)。结论:捏脊疗法可能是通过抑制胃窦平滑肌L-型钙通道Cacnalc亚基表达并降低平滑肌内Ca^(2+)浓度,从而促进胃动力恢复的。Objective:To study the effects of chiropractic therapy on L-type calcium channel and Ca^(2+)concentration in gastric antral smooth muscles,gastric emptying rate and small intestinal propulsion rate in rats with functional dyspepsia(FD).Methods:The rats were divided into blank group,model group and intervention group by random number table method with 11 rats in each group.The FDrat model was established by tail clamping stimulation,regularfeeding and 0℃ normal saline gavage in the model group and intervention group.The intervention group received chiropractic treatment every day,the model group only received restraint under the same conditions as chiropractic treatment every day,and the blank group did not do any intervention.The gastric emptying rate,intestinal propulsion rate and Ca^(2+)concentration of gastric antral smooth muscles were detected.The expression of Cacnalc subunit mRNA and protein of L-type calcium channel in gastric antral smooth muscle was detected in each group.Results:After chiropractic treatment,compared with blank group,the gastric emptying rate and smallintestinal propulsion rate decreased significantly in model group(P<0.05,P<0.01),the concentration of Ca*in smooth muscle increased signifcantly(P<0.01),the mRNA and protein expression level of Cacnalc subunit increased significantly(P<0.05,P<0.01).Compared with model group,the gastric emptying rate and small intestinal propulsion rate decreased significantly in intervention group(P<0.05),the concentration of Ca^(2+)in smooth muscle increased significantly(P<0.01),the protein expression level of Cacnalc subunit increased significantly(P<0.01).Conclusion:Chiropractic therapy may promote the recovery of gastric motility by inhibiting the expression of L-type calcium channel Cacnalc subunit and reducing the concentration of intracellular Ca^(2+)in gastric antral smooth muscles.

关 键 词：捏脊 功能性消化不良 胃动力 平滑肌 钙通道 

分 类 号：R244.1[医药卫生—针灸推拿学]