脉管复康片对血栓闭塞性脉管炎大鼠的疗效及对Th1/Th2免疫平衡的调节  

Therapeutic effect of Maiguan Fukang Tablets on rats with thromboangitis obliterans and its regulating effect on Th1/Th2 immune balance

作　　者：冯夏 王御震 祝艳丹 梁新雨 张亚旭 赵诚 曹烨民 FENG Xia;WANG Yuzhen;ZHU Yandan;LIANG Xinyu;ZHANG Yaxu;ZHAO Cheng;CAO Yemin(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Vascular Surgery,Shanghai TCM-Integrated Hospital,Shanghai University of TCM,Shanghai 200082,China)

机构地区：[1]上海中医药大学,上海201203 [2]上海中医药大学附属上海市中西医结合医院脉管科,上海200082

出　　处：《中华中医药杂志》2023年第11期5489-5494,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82174382);上海市2020年度科技创新行动计划(No.20Z21900200)。

摘　　要：目的:观察脉管复康片对血栓闭塞性脉管炎(TAO)大鼠的疗效及对Th1/Th2免疫平衡的调节作用。方法:SPF级雄性Wistar大鼠24只,随机分为假手术组、TAO模型组、脉管复康片组,脉管复康片组给予脉管复康片0.75g·kg^(-1)·d^(-1)灌胃,假手术组与TAO模型组给予等量纯水,观察各组大鼠体质量及脾脏指数,对各组大鼠患肢形态变化进行评级;使用激光多普勒灌注成像仪扫描下肢血流量变化;ELISA检测血清中干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)、白细胞介素-4(IL-4)表达情况;流式细胞术检测脾脏和腹股沟淋巴结组织中Th1、Th2表达;HE染色观察股动脉和股静脉组织损伤;Western Blot检测股动脉中细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)、TNF-α蛋白表达水平。结果:第14天,与TAO模型组比较,脉管复康片组大鼠体质量显著增加(P<0.01),脾脏指数显著下降(P<0.01),患肢形态变化评级显著降低(P<0.05),患肢血流量显著提高(P<0.01),血清中IFN-γ、TNF-α、IL-4表达水平显著下降(P<0.01),脾脏和腹股沟淋巴结组织中Th1、Th2均显著下降(P<0.01,P<0.05);股动脉组织中VCAM-1、ICAM-1、TNF-α蛋白表达水平显著下降(P<0.01);HE染色显示脉管复康片组大鼠股动脉炎症细胞浸润面积、纤维增生、血栓形成以及股静脉中炎症细胞浸润面积均显著减少(P<0.01)。结论:脉管复康片可改善TAO大鼠股动脉中内皮细胞活化引起的血管炎性损伤、纤维增生以及血栓形成,可能与其调节Th1/Th2免疫平衡有关。Objective:To observe the therapeutic effect of Maiguan Fukang Tablets(MGFK)on thromboangitis obliterans(TAO)rats Therapeutic effect and regulation of Th1/Th2 immune balance.Methods:Twenty four SPF grade male wistar rats were randomly divided into sham operation group,TAO model group and MGFK group.MGFK group was given MGFK 0.75g·kg^(-1)·d^(-1) by gavage,sham operation group and TAO Model group were given the same amount of pure water.The body weight and spleen index of rats in each group were observed,and the morphological changes of affected limbs in each group were rated;Lower extremity blood flow changes were scanned using laser Doppler perfusion imager;Detection of interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α),interleukin-4(IL-4)expression in rat serum by ELISA;The expression of Th1 and Th2 in spleen and Inguinal lymph nodes was detected by flow cytometry;HE stain was used to observe the injury of femoral artery and femoral vein;Detection of intercelluar adhesion molecule(ICAM-1),vascular cell adhesion molecule-1(VCAM-1)and TNF-α protein expression level in femoral artery and vein by Western Blot.Results:Day 14,compared with the TAO group,the weight of rats in MGFK group increased significantly(P<0.01),and the spleen index decreased(P<0.01),the morphological change rating of the affected limb in MGFK group was significantly lower(P<0.05),blood perfusion in the affected limb was significantly increased (P<0.01),the expression level of IFN-γ,TNF-α,IL-4 in serum of TAO rats was reduced(P<0.01),Thl and Th2 cells were decreased significantly in spleen and inguinal lymph nodes(P<0.01,P<0.05),the protein expression levels of VCAM-1,ICAM-1 and TNF-α in the femoral artery tissue was significantly decreased(P<0.01),the area of inflammatory cells infltration,fibrosis,and thrombosis in the femoral artery and the area of inflammatory cell infiltration in the femoral vein were all reduced(P<0.01).Conclusion:MGFK can improve vascular inflammatory injury,fibroplasia and thrombosis caused by endothelial cell ac

关 键 词：血栓闭塞性脉管炎 脉管复康片 TH1/TH2 内皮细胞活化 

分 类 号：R285.5[医药卫生—中药学]