百合植株再生及遗传转化研究进展  

Research Progress in Regeneration and Genetic Transformation of Lily Plants

作　　者：祁宏英[1,2] 葛宵启 徐洪国 吕文玉[1] 刘天阳 李傲淼 王钰钰 QI Hongying;GE Xiaoqi;XU Hongguo;LYU Wenyu;LIU Tianyang;LI Aomiao;WANG Yuyu(College of Life Science,Agriculture and Forestry,Qiqihar University,Qiqihar 161006,China;Key Laboratory of Resistance Gene Engineering and Cold Region Biodiversity Protection in Heilongjiang Province,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006 [2]黑龙江省抗性基因工程与寒地生物多样性保护重点实验室,齐齐哈尔161006

出　　处：《内蒙古农业大学学报（自然科学版）》2023年第3期78-84,共7页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：黑龙江省高等教育教学改革一般项目(SJGY20210944);齐齐哈尔大学教育科学研究项目(GJQTYB202205);齐齐哈尔大学创新创业训练项目(x202210232077);齐齐哈尔大学2022年校级“课程思政”示范课程培育项目(QDJG-2022KCSZ04);黑龙江省教育厅基本科研业务专项项目(145109508,145209514)。

摘　　要：百合是多年生单子叶球根花卉,具有食用、药用及观赏价值。近年来,百合的需求量逐渐加大,育种目标也越来越丰富,百合快速繁育及培育优良新品种一直是研究的热点。传统繁殖方式繁殖系数低且速度慢,通过组织培养技术建立再生体系及遗传转化体系有利于百合的快速繁育及优良品种选育。本文从外植体的选择、培养基类型、器官直接发生途径、器官间接发生途径、体细胞胚状体途径、遗传转化受体及方法的选择等方面概述了百合植株再生及遗传转化的研究进展,为百合高效再生体系及遗传转化体系的建立提供参考依据。Lily is a perennial monocotyledonous bulbous flower with edible,medicinal,and ornamental values.In recent years,the demand for lilies has gradually increased,and breeding objectives have become increasingly diverse.Rapid breeding of lilies and the cultivation of excellent new varieties have always been a research hot spot.The traditional reproduction method has a low coefficient of reproduction and a slow speed.Establishing a regeneration system and genetic transformation system through tissue culture technology is conducive to the rapid breeding and excellent variety selection of lilies.This paper summarized the research progress of lily plant regeneration and genetic transformation from the aspects of explant selection,culture medium type,direct organogenesis,indirect organogenesis,somatic cell embryoid pathway,selection of genetic transformation receptors and methods,providing a reference for the establishment of efficient regeneration system and genetic transformation system of lily.

关 键 词：百合 外植体 组织培养 离体再生 遗传转化 

分 类 号：S682.29[农业科学—观赏园艺]