GAEP:a comprehensive genome assembly evaluating pipeline  

作　　者：Yong Zhang Hong-Wei Lu Jue Ruan 

机构地区：[1]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518120,China [2]State Key Laboratory of Rice Biology and Breeding,China National Rice Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou,Zhejiang 311401,China

出　　处：《Journal of Genetics and Genomics》2023年第10期747-754,共8页遗传学报（英文版）

基　　金：supported by the National Key Research and Development Project Program of China(2022YFC3400300,2019YFE0109600);the China Postdoctoral Science Foundation(2021M701584).

摘　　要：With the rapid development of sequencing technologies,especially the maturity of third-generation sequencing technologies,there has been a significant increase in the number and quality of published genome assemblies.The emergence of these high-quality genomes has raised higher requirements for genome evaluation.Although numerous computational methods have been developed to evaluate assembly quality from various perspectives,the selective use of these evaluation methods can be arbitrary and inconvenient for fairly comparing the assembly quality.To address this issue,we have developed the Genome Assembly Evaluating Pipeline(GAEP),which provides a comprehensive assessment pipeline for evaluating genome quality from multiple perspectives,including continuity,completeness,and correctness.Additionally,GAEP includes new functions for detecting misassemblies and evaluating the assembly redundancy,which performs well in our testing.GAEP is publicly available at https://github.com/zyoptimistic/GAEP under the GPL3.0 License.With GAEP,users can quickly obtain accurate and reliable evaluation results,facilitating the comparison and selection of high-quality genome assemblies.

关 键 词：Genome assembly Assembly quality Assembly metrics Assembly evaluation pipeline Misassembly detection Misassembly breakpoint Assembly redundancy 

分 类 号：R532.21[医药卫生—内科学]