单细胞拉曼光谱仪杂散光抑制研究  

Stray Light Suppression of Single-Cell Raman Spectrometer

作　　者：吕鑫 朱明尧 潘科宇 迟明波 吴一辉 LüXin;Zhu Mingyao;Pan Keyu;Chi Mingbo;Wu Yihui(Key Laboratory of Optical System Advanced Manufacturing Technology,Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,Jilin,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院长春光学精密机械与物理研究所光学系统先进制造技术重点实验室,吉林长春130033 [2]中国科学院大学,北京100049

出　　处：《激光与光电子学进展》2023年第19期188-194,共7页Laser & Optoelectronics Progress

基　　金：国家自然科学基金重大科研仪器研制项目(61727813);吉林省科技发展项目(20200401043GX);白求恩医学工程与仪器中心基金(BQEGCZX2021010);中国科学院科研仪器研制项目(YJKYYQ20210035)。

摘　　要：自发拉曼光谱检测具有宽光谱高分辨但信号较弱的特点,因此杂散光的影响不可忽视。针对自行设计的单细胞拉曼光谱仪光栅转动下系统杂散光产生的机理进行了研究,给出了杂散光抑制方法,特别是多重结构下消除杂散光光学陷阱的设计方法,通过Trace Pro软件仿真验证了其杂散光抑制的有效性,分析结果显示,杂散光抑制后不同波长处杂散辐射比降低了12%~47%,细胞拉曼光谱仪整体杂散光水平低于10^(-6),经杂散光抑制后的光谱仪更加有利于微弱光信号的探测。Spontaneous Raman spectroscopy exhibits a broad spectrum and high resolution but weak signals;thus,the influence of stray light cannot be ignored.In this study,the mechanism of stray light generation in a self-designed singlecell Raman spectrometer system under gyration rotation is investigated.Moreover,the stray light suppression method is established,particularly the design method for eliminating a stray light optical trap in a multi-structure.The effectiveness of stray light suppression is verified using Trace Pro software simulation.The analysis results show that after stray light suppression the stray radiation ratios at different wavelengths decrease by 12%?47%,and the overall stray light level of the cell Raman spectrometer is lower than 10^(-6).The spectrometer,after the stray light suppression,is valuable for detecting weak light signals.

关 键 词：光谱学 单细胞拉曼光谱仪 光学陷阱 TRACEPRO 杂散光抑制 

分 类 号：O439[机械工程—光学工程] O433.4[理学—光学]