艾bZIP基因家族全基因组鉴定及萜类合成调控基因筛选  

Genome-wide identification of bZIP family genes and screening of candidate AarbZIPs involved in terpenoid biosynthesis in Artemisia argyi

作　　者：程泊涵 王梦月 邬兰[1,2] 高冉冉 尹青岗 师玉华[1,2] 向丽[1,2] CHENG Bo-han;WANG Meng-yue;WU Lan;GAO Ran-ran;YIN Qing-gang;SHI Yu-hua;XIANG Li(Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Artemisinin Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所中药鉴定与安全性评估北京市重点实验室,北京100700 [2]中国中医科学院青蒿素研究中心,北京100700

出　　处：《中国中药杂志》2023年第19期5181-5194,共14页China Journal of Chinese Materia Medica

基　　金：中国中医科学院科技创新工程项目(CI2021A04107);中央级公益性科研院所基本科研业务费专项(ZZ16-ND-10-09)。

摘　　要：艾是我国重要的药用植物和经济作物,具有温经散寒、止痛、抗炎、抗过敏等功效。艾叶精油富含挥发性萜类化合物,广泛应用于艾灸及保健品中,市场开发潜力巨大。bZIP转录因子家族在植物中数量众多,广泛参与植物生长发育、胁迫应答和萜类等次生代谢产物合成调控,但艾bZIP家族及其功能鲜有报道。该研究基于艾基因组系统鉴定bZIP家族转录因子,分析其蛋白理化性质、进化关系、保守基序和启动子顺式作用元件等特性,并筛选可能参与萜类合成调控的候选基因。结果显示:从基因组水平共鉴定到156个AarbZIP转录因子,其编码氨基酸为99~618 aa,相对分子质量为11.7~67.8 kDa,理论等电点为4.56~10.16。根据拟南芥bZIP家族分类,AarbZIP蛋白分为12个亚族,同一亚族蛋白具有相似的保守基序。启动子顺式作用元件分析表明AarbZIP家族与光和激素响应密切相关。同源性比对和系统发育分析共筛选出5个可能参与萜类合成调控的AarbZIP基因。qRT-PCR结果表明5个候选AarbZIP基因在艾不同组织中表达存在显著差异,其中AarbZIP29和AarbZIP55在叶片中表达量最高,AarbZIP81、AarbZIP130、AarbZIP150在花蕾中表达量最高。该研究为艾bZIP家族基因的功能研究及其在艾萜类合成中的调控作用解析奠定基础。Artemisia argyi is an important medicinal and economic plant in China,with the effects of warming channels,dispersing cold,and relieving pain,inflammation,and allergy.The essential oil of this plant is rich in volatile terpenoids and widely used in moxi-bustion and healthcare products,with huge market potential.The bZIP transcription factors compose a large family in plants and are involved in the regulation of plant growth and development,stress response,and biosynthesis of secondary metabolites such as terpenoids.However,little is known about the bZIPs and their roles in A.argyi.In this study,the bZIP transcription factors in the genome of A.argyi were systematically identified,and their physicochemical properties,phylogenetic relationship,conserved motifs,and promoter-binding elements were analyzed.Candidate AarbZIP genes involved in terpenoid biosynthesis were screened out.The results showed that a total of 156 AarbZIP transcription factors were identified at the genomic level,with the lengths of 99-618 aa,the molecular weights of 11.7-67.8 kDa,and the theoretical isoelectric points of 4.56-10.16.According to the classification of bZIPs in Arabidopsis thaliana,the 156 AarbZIPs were classified into 12 subfamilies,and the members in the same subfamily had similar conserved motifs.The cis-acting elements of promoters showed that AarbZIP genes were possibly involved in light and hormonal pathways.Five AarbZIP genes that may be involved in the regulation of terpenoid biosynthesis were screened out by homologous alignment and phylogenetic analysis.The qRT-PCR results showed that the expression levels of the five AarbZIP genes varied significantly in different tissues of A.argyi.Specifically,AarbZIP29 and AarbZIP55 were highly expressed in the leaves and AarbZIP81,AarbZIP130,and AarbZIP150 in the flower buds.This study lays a foundation for the functional study of bZIP genes and their regulatory roles in the terpenoid biosynthesis in A.argyi.

关 键 词：艾 bZIP转录因子 基因家族鉴定 实时荧光定量PCR 

分 类 号：S567.239[农业科学—中草药栽培]