烟管头草全草中3个新的二萜类化合物  

Three new diterpenoids from whole herb of Carpesium cernuum

作　　者：冯树慧 张卫青 梁伟[1] 晏晨[1] FENG Shu-hui;ZHANG Wei-qing;LIANG Wei;YAN Chen(People′s Hospital of Anshun City,Anshun 561000,China;Guizhou University of Traditional Chinese Medicine,Guiyang 550002,China)

机构地区：[1]安顺市人民医院,贵州安顺561000 [2]贵州中医药大学,贵州贵阳550002

出　　处：《中国中药杂志》2023年第19期5244-5249,共6页China Journal of Chinese Materia Medica

基　　金：贵州省科技计划项目(黔科合支撑[2022]一般189)。

摘　　要：采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和半制备高效液相色谱等方法对烟管头草Carpesium cernuum全草的化学成分进行研究,通过MS、NMR等现代波谱技术对化合物进行结构鉴定。从烟管头草乙酸乙酯部位分离鉴定了3个新的二萜类化合物,分别为(5Z)-2-oxo-2,10,14-trimethylhexadeca-5,13-diene-11α,18-diol(1)、(2E,10E)-7-[(acetyloxy)methyl]-3,11,15-trimethylhexadeca-2,10,14-triene-1,12α-diol(2)、(2E,6Z)-3,11,15-trimethylhexadeca-2,6,14-triene-1,12α,19-triol(3)。采用MTT法测定化合物1~3对人前列腺癌细胞DU-145、人乳腺癌细胞MCF-7、人肺癌细胞A549的细胞毒活性,结果显示化合物1和3对人乳腺癌细胞MCF-7具有一定的抑制作用,其抑制率分别为45.06%、29.40%。The study investigated the chemical constituents from the whole herb of Carpesium cernuum.Three new diterpenoids were isolated from the whole herb of C.cernuum by column chromatography on silica gel,Sephadex LH-20,and semi-preparative HPLC.Their structures were identified by MS,NMR and other spectral techniques.The isolates were identified as(5Z)-2-oxo-2,10,14-trimethylhexadeca-5,13-diene-11α,18-diol(1),(2E,10E)-7-[(acetyloxy)methyl]-3,11,15-trimethylhexadeca-2,10,14-triene-1,12α-diol(2),(2E,6Z)-3,11,15-trimethylhexadeca-2,6,14-triene-1,12α,19-triol(3),respectively.The cytotoxic activity of compounds 1-3 were investigated with DU-145,MCF-7,and A549 cells by MTT.The results showed that compound 1 and 3 had certain inhibitory effects on MCF-7 cells,with the inhibition rates of 45.06% and 29.40%,respectively.

关 键 词：烟管头草 化学成分 二萜 结构鉴定 细胞毒活性 

分 类 号：R284[医药卫生—中药学]