HPLC-ELSD测定灵芝孢子油中8个甘油三酯类成分的含量  

Assay of 8 Triglycerides in Ganoderma Lucidum Spore Oil by HPLC-ELSD

作　　者：郭原 朱米群 钱一帆 温明皓 陆宇婷 宋敏[2] 杭太俊[2] GUO Yuan;ZHU Miqun;QIAN Yifan;WEN Minghao;LU Yuting;SONG Min;HANG Taijun(Nanjing Zhongke Pharmaceutical Co.,Ltd.,Nanjing 211102,China;Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing 21009,China)

机构地区：[1]南京中科药业有限公司,南京211102 [2]中国药科大学药物分析系,南京210009

出　　处：《药学与临床研究》2023年第5期430-433,共4页Pharmaceutical and Clinical Research

摘　　要：目的:建立灵芝孢子油中甘油三酯类成分含量的高效液相色谱-蒸发光散射检测测定法。方法:采用ODS(100 mm×4.6 mm,2.7μm)色谱柱,以有机混合溶剂乙腈-无水乙醇-甲基叔丁基醚(45∶45∶10)为流动相等度洗脱,采用蒸发光散射检测法,分离了灵芝孢子油中8个主要甘油三酯类成分,以峰面积对数(lg A)为Y,质量浓度对数(lg C)为X进行线性回归并测定含量。结果:方法验证结果表明,各组分峰间的分离度符合要求,它们的蒸发光散射响应均在4.0~200μg·mL^(-1)浓度范围呈现良好的线性关系(r>0.999),平均加样回收率为96.63%~107.53%(RSD<5.0%)。结论:该方法专属性好,分析洗脱时间适宜,定量准确,满足灵芝孢子油中甘油三酯类成分质量控制的要求。Objective:To establish an HPLC-ELSD method for the assay of triglycerides contained in Ganoderma lucidum spore oil.Methods:Separation of 8 main triglycerides in Ganoderma lucidum spore oil was performed on an ODS(100 mm×4.6 mm,2.7μm)column by isocratic elution using acetonitrile-anhy-drous alcohol-methyl tertbutyl ether with the volume ratio of 45∶45∶10 as the mobile phase and evaporative light-scattering detection.They were quantified by a calibration curve method using linear regression algo-rithm of the log peak areas(lgA)as Y to log mass concentrations(lgC)as X.Results:The results of method validation showed that the resolution between the peaks of each component met the requirements,and all their evaporative light scattering responses showed good linear relationship in the mass concentra-tion range of 4.0-200μg·mL^(-1)(r>0.999).The average recoveries were between 96.63%and 107.53%(RSD<5.0%).Conclusion:This method has good specificity,suitable elution time and quantification accu-racy,which can be applied for quality control of triglycerides contents in Ganoderma lucidum spore oil.

关 键 词：灵芝孢子油 甘油三酯 含量测定 高效液相色谱-蒸发光散射检测法 

分 类 号：R927.2[医药卫生—药学]