Dynamic RNA profiles in the small intestinal epithelia of cats after Toxoplasma gondii infection  

作　　者：Bintao Zhai Shi-Chen Xie Jiyu Zhang Jun-Jun He Xing-Quan Zhu 

机构地区：[1]Key Laboratory of Veterinary Pharmaceutical Development,Ministry of Agriculture and Rural Affairs,Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou,730050,Gansu,People’s Republic of China [2]State Key Laboratory for Animal Disease Control and Prevention,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,730046,Gansu,People’s Republic of China [3]Laboratory of Parasitic Diseases,College of Veterinary Medicine,Shanxi Agricultural University,Taigu,030801,Shanxi,People’s Republic of China [4]Research Center for Parasites&Vectors,College of Veterinary Medicine,Hunan Agricultural University,Changsha,410128,Hunan,People’s Republic of China [5]Key Laboratory of Veterinary Public Health of Yunnan Province,College of Veterinary Medicine,Yunnan Agricultural University,Kunming,650201,Yunnan,People’s Republic of China

出　　处：《Infectious Diseases of Poverty》2023年第4期63-82,共20页贫困所致传染病（英文）

基　　金：Project support was kindly provided by the National Key Research and Development Program of China(Grant Nos.2021YFC2300800 and 2021YFC2300802);the National Natural Science Foundation of China(Grant Nos.32172887 and 32102701);the Youth Science and Technology Fund Program of Gansu province(Grant No.23JRRA562);the Innovation Project of Chinese Academy of Agricultural Sciences(Grant No.25-LZIHPS-05);the Agricultural Science and Technology Innovation Program(ASTIP)(Grant No.CAAS-ASTIP-2016-LVRI-03);the Yunnan Expert Workstation(Grant No.202005AF150041);The funding bodies played no role in the design of the study and collection,analysis,and interpretation of data and in writing the manuscript.

摘　　要：Background Felids are the only definitive hosts ofToxoplasma gondii. However, the biological features of the feline small intestine followingT. gondii infection are poorly understood. We investigated the changes in the expression of RNAs (including mRNAs, long non-coding RNAs and circular RNAs) in the small intestinal epithelia of cats followingT. gondii infection to improve our understanding of the life cycle ofT. gondii and cat responses toT. gondii infection.Methods Fifteen cats were randomly assigned to five groups, and the infection groups were inoculated with 600 tissue cysts of theT. gondii Pru strain by gavage. The small intestinal epithelia of cats were collected at 6, 10, 14, and 30 days post infection (DPI). Using high-throughput RNA sequencing (RNA-seq), we investigated the changes in RNA expression. The expression levels of differentially expressed (DE) genes and non-coding RNAs (ncRNAs) identified by RNA-seq were validated by quantitative reverse transcription PCR (qRT-PCR). Differential expression was determined using the DESeq R package.Results In total, 207 annotated lncRNAs, 20,552 novel lncRNAs, 3342 novel circRNAs and 19,409 mRNAs were identified. Among these, 70 to 344 DE mRNAs, lncRNAs and circRNAs were detected, and the post-cleavage binding sites between 725 ncRNAs and 2082 miRNAs were predicted. Using the co-location method, we predicted that a total of 235 lncRNAs target 1044 protein-coding genes, while the results of co-expression analysis revealed that 174 lncRNAs target 2097 mRNAs. Pathway enrichment analyses of the genes targeted by ncRNAs suggested that most ncRNAs were significantly enriched in immune or diseases-related pathways. NcRNA regulatory networks revealed that a single ncRNA could be directly or indirectly regulated by multiple genes or ncRNAs that could influence the immune response of cats. Co-expression analysis showed that 242 circRNAs, mainly involved in immune responses, were significantly associated withT. gondii infection. In contrast, 1352 protein coding RNAs, ma

关 键 词：Toxoplasma gondii Definitive host CAT Small intestinal epithelia Long non-coding RNA Circular RNA MRNA 

分 类 号：R382.5[医药卫生—医学寄生虫学]