马铃薯品种对马铃薯金线虫的抗性评价  

Resistance Evaluation of Different Potato Varieties to Globodera rostochiensis

作　　者：李艳 陈敏 代艳琼 李永青 邓春菊 杨毅娟 杨艳梅 胡先奇 LI Yan;CHEN Min;DAI Yan-qiong;LI Yong-qing;DENG Chun-Ju;YANG Yi-juan;YANG Yan-mei;HU Xian-qi(College of Plant Protection,Yunnan Agricultural University/State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Kunming 650201,China;Zhaotong Municipal Plant Protection and Quarantine Station,Zhaoyang 657500,China;Xuanwei Agricultural Technology Extension Center,Xuanwei 655400,China)

机构地区：[1]云南农业大学植物保护学院/省部共建云南生物资源保护与利用国家重点实验室,云南昆明650201 [2]云南省昭通市植保植检站,云南昭阳657500 [3]云南省宣威市农业技术推广中心,云南宣威655400

出　　处：《江西农业学报》2023年第9期46-54,共9页Acta Agriculturae Jiangxi

基　　金：云南省基础研究专项—青年项目(202301AU070116);2019年中央农业生产救灾(第十批)资金课题(云财农[2020]7号)。

摘　　要：结合根球计数法、平均增殖率法、相对敏感性评价法及酸性品红染色计数法等多种方法,同步开展温室试验与田间试验,接种用金线虫同源,对14个马铃薯品种的金线虫抗性进行了评价。结果表明,供试的14个马铃薯品种中未发现对金线虫免疫的品种,品种Y505、X5表现出稳定的抗性,YS3、HJ01和V7表现出一定的抗性,但可能受环境因素的影响而表现不稳定,其他9个品种不具备抗性。结合试验结果分析了根球计数法、平均增殖率法、相对敏感性评价法各自的优点以及分别单独使用存在的局限性,并提出了多方法比较,温室试验与田间试验同步进行,接种用金线虫同源,开展马铃薯品种对金线虫抗性评价的建议。研究结果为获得精准的、抗性层级清晰的抗性评价、抗性品种的布局和推广、抗性品种培育等提供了有益的参考借鉴。By multiple methods such as root ball counting,average proliferation rate method,relative sensitivity evaluation method,and acid fuchsin staining counting method,greenhouse and field experiments were conducted simultaneously the nematodes used for inoculation were homologous to evaluate the resistance of 14 potato varieties to nematodes.The results showed that none of the 14 potato varieties tested were found to be immune to the nematode.Y505 and X5 showed stable resistance,while YS3,HJ01 and V7 showed some resistance.However,the performance may be unstable due to environmental factors,and the other 9 varieties do not have resistance.Based on the experimental results,the advantages of root ball counting method,average proliferation rate method,and relative sensitivity evaluation method were analyzed,as well as the limitations of using them separately.Suggestions were also proposed for comparing multiple methods,synchronizing greenhouse and field experiments,and conducting resistance evaluation of potato varieties against G.rostochiensis using the same source of nematodes for inoculation.The results can provide useful references for obtaining accurate and clear resistance evaluation results,as well as for the layout,promotion,and cultivation of resistant varieties.

关 键 词：马铃薯金线虫 马铃薯品种 抗性评价 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]