BAFF/BAFF-R单核苷酸多态性与移植肾功能的相关性  

Correlation between BAFF/BAFF-R single nucleotide polymorphism and graft renal function

作　　者：贾晨露 徐海燕[1] 何小舟[1] 徐仁方[1] JIA Chen-lu;XU Hai-yan;HE Xiao-zhou;XU Ren-fang(Urology,Third Affiliated Hospital of Soochow University,Changzhou213003,China)

机构地区：[1]苏州大学附属第三医院泌尿外科,常州213003

出　　处：《现代免疫学》2023年第5期386-392,共7页Current Immunology

摘　　要：为探讨人B细胞激活因子(B cell activating factor belonging to the TNF family,BAFF)及BAFF受体(BAFF-receptor,BAFF-R)单核苷酸多态性(single nucleotide polymorphism, SNP)与肾移植的相关性,随机选取肾移植受者310例和健康体检对照者51例,采集抗凝外周血和血清。选取5个BAFF/BAFF-R SNP,以Taqman qRT-PCR进行基因分型。Hardy-Weinberg平衡检验结果提示基因型分布具有群体代表性。以估算的肾小球滤过率(estimated glomerular filtration rate, eGFR)90 mL/(min·1.73 m^(2))为临界值,将肾移植受者分为eGFR正常组和异常组。Logistic回归分析显示,在杂合遗传模型下BAFF rs9514828 CT基因型携带者移植肾功能异常概率比其他基因型携带者显著升高(OR=1.597, 95%CI 1.012~2.519,P<0.05),与血清可溶性BAFF(soluble BAFF, sBAFF)水平和群体反应性抗体(panel reactive antibody, PRA)阳性率无显著相关性。其余4个SNP基因型在组间的差异无统计学意义。Haploview 4.0软件分析显示,BAFF rs16972194、rs9514828、rs16972197间存在强连锁不平衡。单倍体分析显示,单倍体“GCG”携带者移植肾功能正常概率显著升高(OR=0.381, 95%CI 0.156~0.931,P<0.05)。该研究提示,对肾移植受者来说,BAFF rs9514828 CT基因型可能是移植肾功能异常的风险因素,单倍体GCG可能是移植肾功能正常的保护因素。This study proposes to investigate the association between single nucleotide polymorphism(SNP)of B cell activating factor belonging to the TNF family(BAFF)and BAFF-receptor(BAFF-R)and graft renal function.To this end,anticoagulant peripheral blood and serum were collected from 310 randomly selected following-up renal transplantation recipients and 51 healthy controls.Five BAFF/BAFF-R SNPs were selected and genotyped by Taqman qRT-PCR.The results of the Hardy-Weinberg equilibrium test showed that the distribution of genotypes was representative of the population.Based on the critical value of the estimated glomerular filtration rate(eGFR)of 90 mL/(min·1.73 m^(2)),the recipients were divided into eGFR normal and eGFR abnormal groups.Logistic regression analysis showed that in the heterozygote genetic model,BAFF rs9514828 CT genotype carriers had a significantly higher incidence of abnormal graft kidney function than other genotype carriers(OR=1.597,95%CI 1.012-2.519,P<0.05)whereas it had no significant correlation with serum soluble BAFF(sBAFF)level or positive rate of panel reactive antibody(PRA).The other four SNP genotypes showed no significant difference between groups.Haploview 4.0 software analysis showed that there was a strong linkage disequilibrium between rs16972194,rs9514828,and rs16972197 in BAFF.Haploid analysis showed that haploid“GCG”carriers had a significantly higher chance of normal graft function(OR=0.381,95%CI 0.156-0.931,P<0.05).Altogether,the results suggest that for kidney transplant recipients,BAFF rs9514828 CT genotype may be a risk factor for reduced graft function,and the haploid GCG may be a protective factor in favor of normal graft function.

关 键 词：人B细胞激活因子 人B细胞激活因子受体 单核苷酸多态性 肾移植 

分 类 号：R392.4[医药卫生—免疫学]