冲和膏促进肿疡消散的体内外机制研究  

Study on the mechanism of Chonghe paste promoting the dissipation of swollen lesions in vivo and in vitro

作　　者：杨丽萍 周帅 贾晴 刘善钊 刘钰 YANG Liping;ZHOU Shuai;JIA Qing;LIU Shanzhao;LIU Yu(College of Traditional Chinese Medicine,Inner Mongolia Medical University,Hohhot 010110,China;College of Integrated Traditional Chinese and Western Medicine,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]内蒙古医科大学中医学院,呼和浩特010110 [2]湖南中医药大学中西医结合学院,长沙410208

出　　处：《中国药房》2023年第22期2708-2714,共7页China Pharmacy

基　　金：国家自然科学基金项目(No.82205127);湖南省自然科学基金项目(No.2020JJ5861);内蒙古自治区自然科学基金面上项目(No.2022MS08021)。

摘　　要：目的 探究冲和膏促进肿疡消散的机制。方法 采取打孔法检测冲和膏对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、白色葡萄球菌、肺炎链球菌的抑菌作用。采用皮下注射金黄色葡萄球菌的方法建立皮下软组织感染大鼠模型,考察冲和膏(复方多黏菌素B软膏为阳性对照)干预14 d对其皮下软组织病理学变化,皮下软组织中Ⅰ型胶原、Ⅲ型胶原、基质金属蛋白酶2(MMP-2)、MMP-9蛋白表达量,血清中转化生长因子β(TGF-β)、碱性成纤维细胞生长因子(bFGF)含量的影响。结果 冲和膏对上述4种细菌(肺炎链球菌除外)均有不同程度的抑菌效果,其中对金黄色葡萄球菌的抑菌效果最好。与模型组相比,给药第7天,冲和膏组大鼠胶原纤维排列有序,脓液消散较快,Ⅰ型胶原、Ⅲ型胶原蛋白的表达量和TGF-β、bFGF含量均显著升高,MMP-2、MMP-9蛋白的表达量均显著降低(P<0.05);给药第14天,冲和膏组大鼠胶原沉积明显,皮下各附属器逐渐形成,Ⅰ型胶原、Ⅲ型胶原蛋白的表达量和TGF-β、bFGF含量均显著降低,MMP-2、MMP-9蛋白的表达量均显著升高(P<0.05)。结论 冲和膏具有抑菌作用,可能通过调控胶原纤维的合成与降解,增加脓肿组织中bFGF、TGF-β的分泌,从而起到促进肿疡消散的作用。OBJECTIVE To explore the mechanism of Chonghe paste promoting the dissipation of swollen lesions.METHODS The bacteriostatic effects of Chonghe paste against Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa,Staphylococcus albus and Streptococcus pneumoniae were detected by punching method.The subcutaneous soft tissue infection model of rats was established by subcutaneous injection of S.aureus.The effects of 14 d intervention of Chonghe paste(Compound polymyxin B ointment as positive control)on the pathological changes of subcutaneous soft tissue,the protein expressions of typeⅠcollagen,typeⅢcollagen,matrix metalloproteinase-2(MMP-2)and MMP-9 in subcutaneous soft tissue,and the contents of transforming growth factor-β(TGF-β)and basic fibroblast growth factor(bFGF)in serum were investigated.RESULTS Chonghe paste had varying degrees of bacteriostatic effect on the above 4 bacteria(except for S.pneumoniae),especially on S.aureus.Compared with the model group,on the 7th day of treatment,collagen fibers in the Chonghe paste group were arranged in an orderly manner,pus dissipated faster;the protein expressions of typeⅠand typeⅢcollagen and the contents of TGF-βand bFGF were up-regulated significantly,while protein expressions of MMP-2 and MMP-9 were decreased significantly(P<0.05).On the 14th day of administration,collagen deposition was obvious in the Chonghe paste group,subcutaneous appendages gradually formed;the protein expressions of typeⅠand typeⅢcollagen and the contents of TGF-βand bFGF were down-regulated significantly,while the protein expressions of MMP-2 and MMP-9 were increased significantly(P<0.05).CONCLUSIONS Chonghe paste has the bacteriostatic effect and may play a role in promoting the dissipation of swollen lesions by regulating the formation and decomposition of fibrin and increasing the secretion of bFGF and TGF-β.

关 键 词：冲和膏 肿疡 抑菌 作用机制 

分 类 号：R965[医药卫生—药理学] R261[医药卫生—药学]