基于表面增强拉曼光谱的果蔬中多菌灵和噻菌灵的快速检测  

Rapid Detection of carbendazim and thiabendazole in vegetables and fruits by Surface-Enhanced Raman Spectroscopy

作　　者：刘文静 潘葳 林惠真 陈宏炬 严绍德 Liu Wen-Jing;Pan Wei;Lin Hui-zhen;Chen Hong-ju;Yan Shao-de(Institute of Agricultural Quality Standards and Testing Technology Research,Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Qualitiy&Safety,Fuzhou,Fujian 350003,China;Xiamen PERSer Nanotechnology Co.,Ltd,Xiamen,,Fujian 361021,China;Xiamen Assessment Service Center of Quality and Technology,Xiamen,,Fujian 361021,China)

机构地区：[1]福建省农业科学院农业质量标准与检测技术研究所/福建省农产品质量安全重点实验室,福建福州350003 [2]厦门市普识纳米科技有限公司,福建厦门361021 [3]厦门市质量技术评审服务中心,福建厦门361021

出　　处：《福建分析测试》2023年第6期1-6,21,共7页Fujian Analysis & Testing

基　　金：福建省属公益类科研院所基本科研专项项目(2021R1022008);福建省“5511”协同创新工程项目(XTCXGC2021020);福建省农业科学院创新团队项目(CXTD2021011-1)。

摘　　要：建立了基于表面增强拉曼光谱(Surface-enhanced Raman scattering,SERS)检测果蔬中多菌灵和噻菌灵的方法。以果蔬农产品为研究对象,通过筛选SERS增强试剂、优化提取溶剂和反应时间实验得到最佳检测条件。方法采用纳米金溶胶作为SERS增强试剂,纯水作为提取溶剂,3min内判读实验结果。对多菌灵的主要拉曼谱峰进行归属,以616 cm^(-1)、731 cm^(-1)、1006 cm^(-1)、1223 cm^(-1)、1263 cm^(-1)、1316 cm^(-1)和1370 cm^(-1)作为多菌灵的定性特征峰,其中616 cm^(-1)作为定量特征峰,该处多菌灵溶液拉曼特征峰强度与浓度之间的线性范围为0.1~20 mg/L,R^(2)为0.9645,加标回收率为82.5%~105.0%,RSD值为2.1%~5.3%,方法检出限为0.2 mg/kg;对噻菌灵的主要拉曼谱峰进行归属,以786 cm^(-1)和1012 cm^(-1)拉曼谱峰作为噻菌灵的定性特征峰,其中786 cm^(-1)作为定量特征峰,噻菌灵溶液拉曼特征峰强度与浓度之间的线性范围为0.2~50mg/L,R^(2)值为0.9846,加标回收率为88.0%~98.0%,RSD值为2.1%~4.7%,方法检出限为0.5mg/kg。该方法准确、快速、稳定,可实现多菌灵和噻菌灵在农产品中现场快速检测的要求。A rapid method for detection of carbendazim and thiabendazole residues in vegetables and fruits by Surface-en⁃hanced Raman spectroscopy(SERS)was established.The best detection conditions were obtained by screening of enhance⁃ment reagent,optimizing extraction solvents and reaction time using fruit and vegetable products as the research objects.The gold nanoparticles was used as the SERS enhancement reagent,pure water as the extraction solvent,and the experimental results were interpreted within 3 min.SERS spectra were obtained and the characteristic bands were assigned,the qualita⁃tive characteristic peaks of carbendazim were 616 cm^(-1)、731 cm^(-1)、1006 cm^(-1)、1223 cm^(-1)、1263 cm^(-1)、1316 cm^(-1) and 1370 cm^(-1),with 616 cm^(-1) as the quantitative characteristic peak,the linear range between Raman peak intensity and concentration of carbendazim solution was 0.1-20 mg/L with R^(2) was 0.9645,and the recovery between 82.5%-105.0%,the RSD value be⁃tween 2.1%-5.3%,and the limit of detection was 0.2 mg/kg;the qualitative characteristic peaks of thiabendazole were 786 cm^(-1) and 1012 cm^(-1),with 786 cm^(-1) as the quantitative characteristic peak,the linear range between Raman peak intensity and concentration of carbendazim solution was 0.2-50 mg/L with R^(2) was 0.9846,and the recovery between 82.5%-105.0%,the RSD value between 2.1%-4.7%,and the limit of detection was 0.5 mg/kg.The method is accurate,rapid,stable,and can achieve the requirements of on-site rapid detection of carbendazim and thiamethoxam in agricultural products.

关 键 词：表面增强拉曼光谱 多菌灵 噻菌灵 快速检测 

分 类 号：O657.37[理学—分析化学]