羌药胃草质量评价研究  

Quality Evaluation for Qiang Medicine Gastric Grass

作　　者：谭承佳[1,2] 田徽 黄御莲[1,2] 李玉莲 刘容西 马晓燕 TAN Chengjia;TIAN Hui;HUANG Yulian;LI Yulian;LIU Rongxi;MA Xiaoyan(School of Life Science and Technology,Mianyang Teachers'College,Mianyang,Sichuan 621000;Mianyang Key Laboratory of Quality Control and Evaluation of Traditional Chinese Medicine,Mianyang,Sichuan 621006;Zhongqiang Medical Hospital of Beichuan Qiang Autonomous County,Beichuan,Sichuan 622750)

机构地区：[1]绵阳师范学院生命科学与技术学院,四川绵阳621000 [2]绵阳市中药质量控制与评价重点实验室,四川绵阳621006 [3]北川羌族自治县中羌医医院,四川北川622750

出　　处：《绵阳师范学院学报》2023年第11期61-67,共7页Journal of Mianyang Teachers＇ College

基　　金：四川省科技计划项目(2020YFS0539)。

摘　　要：为了建立羌药胃草质量评价方法,采用显微法、TLC法定性鉴别牡荆素、异牡荆素,参照2020年版《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物含量,采用HPLC法测定牡荆素含量、UV-Vis测定总黄酮含量.结果显示,显微特征明显,可见方晶、非腺毛、梯纹导管、木纤维、晶鞘纤维、淀粉粒等;TLC斑点清晰,分离度好;6批样品水分、总灰分、酸不溶性灰分、浸出物含量分别为1.50%~1.83%、4.63%~4.80%、1.16%~1.21%、15.64%~16.58%,牡荆素在0.101~0.505μg范围内线性关系良好(r=0.9999),平均加样回收率为100.69%,RSD为2.56%,平均含量为0.1149 mg/g;总黄酮(以芦丁计)在0.006~0.03 mg/mL范围内,线性关系良好(r=0.9996),平均加样回收率为97.2%,RSD为1.26%,平均含量为2.0641 mg/g.结论:该方法简便可靠,可用于羌药胃草的质量评价.AIM:establish the method of quality evaluation for Qiang medicine gastric grass.METHODS:The qualitative identification was performed by microscopic and TLC.The contents of water,total ash,acid insoluble ash and extract were determined according to Chinese Pharmcopoeia(2020 edition)method.HPLC was adopted in the content determination of vitexin.RESULTS:The microscopic feature were obvious,square crystals,non-glandular hairs,trapezoidal ducts,wood fibers,sheath fibers,starch granules could be observed.The TLC spots were clear with good resolution.The contents of water,total ash,acid-insoluble ash and extract were 1.50%-1.83%、4.63%-4.80%、1.16%-1.21%、15.64%-16.58%.In six batches of samples,respectively vitexin and total flavonoids were showed good linear relationships within their own ranges(r=0.9999 and r=0.9996),whose average recoveries were 100.69% and 97.2% with RSD of 2.56% and 1.26%,The average content were 0.1149 mg/g and 2.0842 mg/g,respectively.CONCLUSION:This reasonable and feasible method can be used for quality evaluation of the Qiang medicine"Wei Cao"grass.

关 键 词：胃草 牡荆素 异牡荆素 总黄酮 显微法 

分 类 号：R282.6[医药卫生—中药学]