携带松材线虫对松墨天牛成虫肠道和气管细菌的影响  

Effects of carrying Bursaphelenchus xylophilus on the bacteria in the gut and trachea of adult Monochamus alternatus(Coleoptera:Cerambycidae)

作　　者：顾宇彤 朱浩诚 常悦 周娇 樊建庭 赵莉蔺[2,3,4] GU Yu-Tong;ZHU Hao-Cheng;CHANG Yue;ZHOU Jiao;FAN Jian-Ting;ZHAO Li-Lin(National and Local Joint Engineering Laboratory for Biopesticide Efficient Preparation Technology,School of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,China;State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China;CAS Center for Excellence in Biotic Interactions,Beijing 100049,China)

机构地区：[1]浙江农林大学林业与生物技术学院,生物农药高效制备技术国家地方联合工程实验室,杭州311300 [2]中国科学院动物研究所,农业虫害鼠害综合治理研究国家重点实验室,北京100101 [3]中国科学院大学,北京100049 [4]中国科学院生物互作卓越创新中心,北京100049

出　　处：《昆虫学报》2023年第10期1319-1328,共10页Acta Entomologica Sinica

基　　金：国家自然科学基金面上项目(NSFC31970466);农业虫害鼠害综合治理研究国家重点实验室开放课题(IPM2111)。

摘　　要：【目的】探究被松墨天牛Monochamus alternatus携带的松材线虫Bursaphelenchus xylophilus对松墨天牛肠道和气管细菌的影响。【方法】野外采集携带松材线虫的松墨天牛成虫后,取出完整肠道和气管进行细菌总DNA抽提后进行16S rDNA基因测序并拼接,并利用生物信息学方法分析松墨天牛成虫肠道和气管细菌组成、结构、丰度和多样性。【结果】携带松材线虫的松墨天牛成虫肠道和气管细菌菌群共检测到15门26纲66目110科201属296种,可操作分类单元(operational taxonomic unit,OTU)数目为444。携带松材线虫的松墨天牛成虫比未携带松材线虫的松墨天牛成虫肠道优势细菌菌群变化不显著,均为变形菌门(Proteobacteria)肠杆菌目(Enterobacterales);携带松材线虫的松墨天牛成虫气管优势细菌菌群为变形菌门肠杆菌目,未携带松材线虫的松墨天牛成虫气管优势细菌菌群为厚壁菌门(Firmicutes)乳杆菌目(Lactobacillales)。携带松材线虫的松墨天牛成虫较未携带松材线虫的松墨天牛成虫气管细菌多样性和丰度升高,细菌群落结构显著不同;携带松材线虫的松墨天牛成虫肠道和气管中沙雷氏菌属Serratia和肠杆菌属Enterobacter富集。【结论】携带松材线虫后对松墨天牛成虫气管细菌菌群组成、结构、丰度和多样性都有显著影响,携带松材线虫的松墨天牛成虫气管中的沙雷氏菌属和肠杆菌属可能与松材线虫-松墨天牛复合体有紧密的联系。在携带松材线虫后,松墨天牛成虫气管中沙雷氏菌属增加,可能与松墨天牛气管免疫受抑制有关。本研究为解析松墨天牛肠道和气管细菌多样性提供了理论基础,为防控松材线虫提供了新的方向。【Aim】To investigate the effects of Bursaphelenchus xylophilus in Monochamus alternatus on the bacteria in the gut and trachea of M.alternatus.【Methods】The whole gut and trachea were isolated from the collected M.alternatus adults carrying B.xylophilus in the field to extract DNA for sequencing and splicing of the 16S rDNA gene.The composition,structure,abundance and diversity of bacteria in the gut and trachea of M.alternatus adults carrying B.xylophilus were analyzed by bioinformatics methods.【Results】A total of 15 phyla,26 classes,66 orders,110 families,201 genera,296 species and 444 operational taxonomic units(OTUs)of bacterial flora in the gut and trachea of M.alternatus adults carrying B.xylophilus were detected.There was no significant change in the dominant bacterial flora in the gut between M.alternatus adults carrying B.xylophilus and M.alternatus adults without carrying B.xylophilus,and their dominant bacterial flora were Proteobacteria and Enterobacterales.Proteobacteria and Enterobacterales were the dominant bacterial flora in the trachea of M.alternatus adults carrying B.xylophilus,while Firmicutes and Lactobacillales were the dominant bacterial flora in the trachea of M.alternatus adults without carrying B.xylophilus.The diversity and abundance of bacteria in the trachea of M.alternatus adults carrying B.xylophilus were higher than those of M.alternatus adults without carrying B.xylophilus,and the bacterial community structure in the trachea of M.alternatus adults carrying B.xylophilus was significantly different from that of M.alternatus adults without carrying B.xylophilus.Serratia and Enterobacter were enriched in the gut and trachea of M.alternatus adults carrying B.xylophilus.【Conclusion】The introduction of B.xylophilus has significant effects on the composition,structure,abundance and diversity of bacterial flora in the trachea of M.alternatus adults.Serratia and Enterobacter in the trachea of M.alternatus adults may be closely related to the B.xylophilus-M.alternatus complex.T

关 键 词：松墨天牛 松材线虫 肠道细菌 气管细菌 细菌多样性 

分 类 号：Q965.8[生物学—昆虫学]