黑水虻对金霉素菌渣的降解调控分析  

Degradation regulation of chlortetracycline residue by the black soldier fly,Hermetia illucens(Diptera:Stratiomyidae)

作　　者：王会 郑朝中 陈玉梁 王飞[1] 王雷雨 章启慧 黄俊魁 赵浚丞 WANG Hui;ZHENG Chao-Zhong;CHEN Yu-Liang;WANG Fei;WANG Lei-Yu;ZHANG Qi-Hui;HUANG Jun-Kui;ZHAO Jun-Cheng(Agricultural Products Processing Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524001,China;College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang 330045,China;Sichuan Yangyun Biotechnology Group Limited Company,Chengdu 610000,China)

机构地区：[1]中国热带农业科学院农产品加工研究所,湛江524001 [2]江西农业大学生物科学与工程学院,南昌330045 [3]四川扬运生物科技集团有限公司,成都610000

出　　处：《昆虫学报》2023年第10期1354-1361,共8页Acta Entomologica Sinica

基　　金：海南省自然科学基金青年基金项目(321QN304,521QN0930,323QN290);中国热带农业科学院2022年科技“揭榜挂帅”项目(1630122022008);中国热带农业科学院基本科研业务费专项项目(1630122022014,1630122023007)。

摘　　要：【目的】明确黑水虻Hermetia illucens对金霉素菌渣的降解调控作用。【方法】选用同一天收集的黑水虻虫卵培育至3日龄幼虫,转入麦麸与金霉素菌渣不同质量配比(麦麸∶金霉素菌渣=1∶0,1∶1,2∶1,5∶1,10∶1和20∶1)混合养殖料中饲养,测定不同日龄黑水虻幼虫体长与体重,依据幼虫和养殖残料中金霉素含量计算不同日龄金霉素降解率。【结果】金霉素菌渣在混合养殖料中的含量对黑水虻幼虫体长和体重等生物学特征具有显著影响,且对不同日龄黑水虻幼虫对金霉素降解率产生显著影响。麦麸与金霉素菌渣不同质量配比混合养殖料饲养的黑水虻幼虫体长与体重在11日龄前随着日龄增加逐渐增加,13日龄时体长与体重出现下降趋势。麦麸与金霉素菌渣5∶1,10∶1和20∶1质量配比混合养殖料饲养时幼虫体长和体重大于对照(纯麦麸饲养组)。通过计算不同质量配比养殖料饲养的不同日龄黑水虻幼虫对金霉素降解率,发现金霉素初始含量是影响降解率的重要因素,麦麸与金霉素菌渣5∶1质量配比混合养殖料饲养的7,9,11和13日龄黑水虻幼虫对金霉素降解率分别为39.21%,68.94%,66.53%和68.26%,显著高于麦麸与金霉素菌渣10∶1和20∶1质量配比组。【结论】黑水虻能够减量化处理金霉素菌渣,养殖料中金霉素的初始含量对黑水虻幼虫生物学特征和对金霉素的降解率具有显著影响。【Aim】To clarify the degradation regulation of chlortetracycline residue by the black soldier fly,Hermetia illucens.【Methods】The eggs of H.illucens collected on the same day were cultured to the 3-day-old larvae,then transferred to the mixed diets of wheat bran and chlortetracycline residue in different mass ratios(wheat bran∶chlortetracycline residue=1∶0,1∶1,2∶1,5∶1,10∶1 and 20∶1),and the body length and body weight of different day-old larvae were measured,meanwhile,the degradation rate of chlortetracycline by different day-old larvae were calculated based on the chlortetracycline contents in H.illucens larvae and diet residues.【Results】The content of chlortetracycline residue in the mixed diets exhibited significant effects on the biological characteristics such as body length and body weight of H.illucens larvae,and the degradation rate of chlortetracycline by different day-old larvae of H.illucens.The body length and body weight of H.illucens larvae reared with the mixed diets of wheat bran and chlortetracycline residue in different mass ratios were increased with the day-old age of larvae until 11-day-old,and then decreased at the 13-day-old larval stage.The body length and body weight of H.illucens larvae reared with the mixed diets of wheat bran and chlortetracycline residue in the mass ratios of 5∶1,10∶1 and 20∶1 were bigger than those of the control group cultured with the pure wheat bran.The initial content of chlortetracycline had a significant effect on the degradation rate of chlortetracycline by different day-old larvae of H.illucens through the comparison of degradation rates of chlortetracycline by different day-old larvae of H.illucens reared with the mixed diets of wheat bran and chlortetracycline residue in different mass ratios.The degradation rates of chlortetracycline by the 7-,9-,11-and 13-day-old larvae reared with the mixed diets of wheat bran and chlortetracycline residue in the mass ratio of 5∶1 were 39.21%,68.94%,66.53% and 68.26%,respectively,signific

关 键 词：黑水虻 金霉素菌渣 降解率 生长发育 减量化 

分 类 号：Q965.8[生物学—昆虫学]