基于脑-肠轴探讨电针与双歧杆菌对单纯性肥胖大鼠肠源性信号物质及脂代谢的影响  

To investigate the effects of electroacupuncture and bifidobacterium on intestinal signaling substances and lipid metabolism in rats with simple obesity based on brain-gut axis

作　　者：颉媛媛 钱虹宇 谢顺铠 许婧[1] Xie Yuanyuan;Qian Hongyu;Xie Shunkai;Xu Jing(Dept of Cosmetology,ChengDu University of TCM,Chengdu 610075)

机构地区：[1]成都中医药大学美容教研室,成都610075

出　　处：《安徽医科大学学报》2023年第11期1841-1845,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81774440)。

摘　　要：目的观察电针与双歧杆菌对单纯性肥胖大鼠生存状态、肠源性信号物质水平及脂代谢的影响,并比较其优效性。方法购入体质量40~60 g范围内的4周龄SPF级SD大鼠60只,10只(雌雄各半)予普通饲料设为空白组,50只(雌雄各半)予高脂饲料制备单纯性肥胖大鼠模型,8周后高脂组造模成功的30只肥胖大鼠随机分为肥胖模型组、电针组、双歧杆菌组,每组10只(雌雄各半),治疗组分别予电针、双歧杆菌溶液灌胃治疗4周,实验期间固定时间称取大鼠体质量;治疗周期结束后采用酶联免疫吸附(ELISA)法检测肥胖大鼠5-羟色胺(5-HT)、组胺(HIS)水平;采用银染色法检测肠嗜铬细胞(ECC)水平;采用生化法检测三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)水平。结果干预后,电针组与双歧杆菌组较模型组大鼠体质量、结肠ECC、结肠与下丘脑5-HT、HIS、血清TC、TG、LDL水平降低,差异有统计学意义(P<0.05),血清HDL水平虽升高,但差异无统计学意义,且电针组较双歧杆菌组各指标更接近于空白组。结论电针对单纯性肥胖大鼠疗效优于双歧杆菌。电针可能是通过调整单纯性肥胖大鼠肠源性信号物质刺激下丘脑机体做出反应减少摄食、加快脂代谢,减缓肥胖。Objective To observe the effects of electroacupuncture and bifidobacterium on the survival state,the level of intestinal signaling substances and lipid metabolism levels in rats with simple obesity,and to compare their efficacy.Methods Sixty 4-week-old SPF SD rats with the body weight ranging from 40 to 60 g were purchased,10(half male and half female)were given ordinary diet as blank group,and 50(half male and half female)were given high fat diet to prepare simple obese rat model.After 8 weeks,30 obese rats successfully established in high fat group were randomly divided into obesity model group,electroacupuncture group and bifidus group,with 10 rats in each group(half male and half female).The treatment group was treated with electroacupuncture and bifidobacterium solution for 4 weeks,respectively,and the body mass of the rats was measured at a fixed time during the experiment.After the treatment cycle,the level of enterochromaffin cell(ECC)in colon was detected by silver staining,the levels of 5-hydroxytryptamine(5-HT),histamine(HIS)in obese rats were detected by ELISA,the levels of total cholesterol(TC),triglyceride(TG),low density lipoprotein(LDL)and high density lipoprotein(HDL)were detected by biochemical method.Results After intervention,compared with model group,body weight,colon and hypothalamus 5-HT,HIS,colon ECC,serum TC,TG and LDL levels decreased in electroacupuncture group and bifidobacterium group,with statistical significance(P<0.05),although the serum HDL levels were increased,the difference was not statistically significant.And the indexes of the electroacupuncture group were more similar to those of the blank group than those of the bifidobacterium group.Conclusion The effect of electrotherapy on simple obesity rats is better than bifidobacterium.Electroacupuncture may stimulate the hypothalamus to reduce food intake,accelerate lipid metabolism and slow down obesity by adjusting the intestinal signaling substances in rats with simple obesity.

关 键 词：脑-肠轴 电针 双歧杆菌 单纯性肥胖 肠源性信号物质 脂代谢 

分 类 号：R245.31[医药卫生—针灸推拿学]