抑制阴道加德纳菌的乳杆菌的筛选及其抑菌机制的研究  

Screening of Lactobacillus that inhibit Gardnerella vaginalis and preliminary study on its bacteriostatic mechanism

作　　者：张瑞[1] 马爱昕 王君竹 李昶[1] 尹海旭 白会会 刘朝晖 Zhang Rui;Ma Aixin;Wang Junzhu;Li Chang;Yin Haixu;Bai Huihui;Liu Zhaohui(Dept of Obstetrics and Gynecology,Peking University First Hospital,Beijing 100034;School of Government Management,Big Data and Public Policy Laboratory,Peking University,Beijing 100091;Dept of Obstetrics and Gynecology,Beijing Obstetrics and Gynecology Hospital,Beijing 100006)

机构地区：[1]北京大学第一医院妇产科,北京100034 [2]北京大学政府管理学院大数据与公共政策实验室,北京100091 [3]北京妇产医院妇产科,北京100006

出　　处：《安徽医科大学学报》2023年第11期1962-1965,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81471420);教育部博士点新教师基金(编号:20130001120051)。

摘　　要：目的筛选对阴道加德纳菌增殖有抑制功能的阴道乳杆菌菌株,探讨阴道乳杆菌菌株抑制加德纳菌的可能机制,为开发用于细菌性阴道病(BV)治疗的益生菌菌株提供先决条件。方法分别从BV患者和健康女性阴道分泌物中分离阴道加德纳菌菌株和乳杆菌菌株,通过spot on lawn法筛选有抑制阴道加德纳菌增殖的乳杆菌菌株,用双层牛津杯法对比乳杆菌菌液、上清液、菌体对阴道加德纳菌菌株抑制作用的差别。结果从20例健康女性阴道分泌物中获得62株阴道乳杆菌菌株,分属于6个菌种,其中18株有抑制阴道加德纳菌的作用。通过对18株有抑菌效应的乳杆菌菌株的不同成份(菌液、菌体、上清液)研究表明,其菌液、菌体和上清液均有抑菌效应,菌液的抑菌效应明显大于上清液组。上清液经过蛋白酶K处理之后,8份标本的抑菌效果消失,10份标本的抑菌效果明显减弱。结论该研究共纯化和鉴定阴道乳杆菌菌株62株,其中18株具有抑制阴道加德纳菌增殖的功能。产生蛋白类抑菌物质可能参与了阴道乳杆菌抑制阴道加德纳菌增殖的机制。Objective To select and obtain vaginal Lactobacillus strains with inhibitory effect on Gardnerella vaginalis,and to explore the possible mechanism of inhibition of Gardnerella vaginalis by Lactobacillus vaginalis strains,so as to provide a prerequisite for the development of dominant strains for human disease treatment.Methods Gardnerella vaginalis and vaginal Lactobacillus strains were isolated from vaginal secretions of patients with bacterial vaginosis(BV)and healthy women,respectively.The Lactobacillus strains with the ability to inhibit the proliferation of Gardnerella vaginalis were screened by spot on lawn.Double agar diffusion method was used to compare the inhibitory effects of Lactobacillus solutions,cell-free culture supernatants(CFCs)and bacteria on Gardnerella vaginalis.Results Sixty-two strains of vaginal Lactobacillus strains were isolated and purified from vaginal secretions of 20 healthy women,belonging to six species.Three strains of Gardnerella vaginalis were isolated from vaginal secretions of 10 patients with bacterial vaginosis.Among the 62 strains of vaginal Lactobacillus,18 strains could inhibit the proliferation of Gardnerella vaginalis.The vaginal Lactobacillus solutions,the CFCs,and bacteria all had inhibitory effect on Gardnerella vaginalis.The inhibitory effects of vaginal Lactobacillus solutions were higher than those of the CFCs.After the CFCs were treated with proteinase K,the inhibitory effect of eight samples disappeared,and that of 10 samples weakened obviously.Conclusion In this paper,62 vaginal Lactobacillus strains are purified and identified,of which 18 strains can inhibit the proliferation of Gardnerella vaginalis.The production of antimicrobial protein may be involved in the mechanism that vaginal Lactobacillus inhibits the proliferation of Gardnerella vaginalis.

关 键 词：抑菌功能 抑菌蛋白 阴道乳杆菌 阴道加德纳菌 细菌性阴道病 

分 类 号：R711.31[医药卫生—妇产科学]