希瓦氏菌分泌黄素对间硝基苯磺酸钠厌氧生物还原影响  

Effect of secreted flavins by Shewanella on anaerobic bio-reduction of sodium 3-nitrobenzene sulfonate

作　　者：洪忠强 吕红[1] 王晓磊 周集体[1] HONG Zhongqiang;LUHong;WANG Xiaolei;ZHOU Jiti(Key Laboratory of Industrial Ecology and Environmental Engineering,Ministry of Education,School of Environmental Science and Technology,Dalian University of Technology,Dalian 116024,China)

机构地区：[1]大连理工大学环境学院工业生态与环境工程教育部重点实验室,辽宁大连116024

出　　处：《大连理工大学学报》2023年第6期560-566,共7页Journal of Dalian University of Technology

基　　金：国家自然科学基金资助项目(221706027).

摘　　要：以Shewanella sp.RQs-106为模式菌株,研究了细胞浓度、间硝基苯磺酸钠(3-NBS)浓度和氧化石墨烯(GO)对3-NBS生物还原速率及细胞分泌黄素类物质的影响,并揭示了胞外黄素在3-NBS厌氧生物还原中的作用.结果表明,以乳酸钠为电子供体,细胞分泌的黄素类物质会随着3-NBS浓度的增大而增加.黄素腺嘌呤二核苷酸转运蛋白基因(bfe)的缺失,使黄素分泌量减少22.8%,3-NBS的生物还原速率下降55.4%;添加20mg/LGO的体系中,胞外黄素对3-NBS直接还原的贡献率为33.7%,参与GO介导下的3-NBS还原的贡献率为14.7%.上述研究表明,黄素参与了3-NBS的厌氧生物还原,并在胞外电子传递中起到了重要作用.Using Shewanella sp.RQs-106 as a model strain,the effects of cell concentration,sodium 3-nitrobenzene sulfonate(3-NBS)concentration and graphene oxide(GO)on the bio-reduction rate of 3-NBS and secreted flavins are investigated,and the role of extracellular flavins during the anaerobic bio-reduction of 3-NBS is revealed.The results show that the concentration of the secreted flavins increases with the increase of 3-NBS concentration using sodium lactate as an electron donor.The deletion of flavin adenine dinucleotide transporter gene(bfe)results in 22.8%and 55.4%of decreases in the amount of the secreted flavins and the bio-reduction rate of 3-NBS,respectively.In 20 mg/L GO-supplemented system,the contributions of extracellular flavins to direct and GO-mediated reduction of 3-NBS are 33.7%and 14.7%,respectively.The above results show that the secreted flavins participate in 3-NBS anaerobic bio-reduction and play an important role in extracellular electron transfer.

关 键 词：Shewanella sp.RQs-106 间硝基苯磺酸钠 黄素 胞外电子传递 氧化石墨烯 

分 类 号：X53[环境科学与工程—环境工程]