白及中的化学成分鉴定及对金葡菌的抑制机理研究  

Study on the chemical constituents from Bletilla striata and its antibacterial mechanism against Staphylococcus aureus

作　　者：罗芳 俞昳 张琳晗 陆梅芬 陈小明[1] LUO Fang;YU Yi;ZHANG Lin-han;LU Mei-fen;CHEN Xiao-ming(College of Chemical and Biological Engineering,Hunan University of Science and Engineering,Yongzhou 425199,China)

机构地区：[1]湖南科技学院化学与工程学院,永州425199

出　　处：《天然产物研究与开发》2023年第11期1894-1901,共8页Natural Product Research and Development

基　　金：湖南省自然科学基金青年项目(2019JJ50197)。

摘　　要：研究白及中的化学成分及对金葡菌的抑制机理。对白及成分进行色谱分离纯化,通过NMR对化合物进行结构鉴定,并用微量肉汤稀释法测试化学成分对金黄色葡萄球菌的抗菌作用。通过考察金黄色葡萄球菌的生长曲线、胞外碱性磷酸酶含量、DNA泄漏程度、细胞壁和细胞膜的完整性的变化探究白及中活性成分的抑菌机制。本研究从该植物中分离鉴定出10个化合物,分别为shancidin(1)、coelonin(2)、lusianthridin(3)、orchinol(4)、isoshancidin(5)、blestriarene A(6)、(E)-3-(4-hydroxyphenyl)acrylic acid(7)、3-phenylpropanoicacid(8)、ethyl 2-(4-hydroxyphenyl)acetate(9)、ethyl 2-(4-ethylphenyl)acetate(10)。其中,化合物7~10为首次从该植物种中分离得到。此外,化合物6有着显著的抑菌效果,最小抑菌浓度在10μg/mL(阳性药物阿莫西林0.8μg/mL),它通过破坏金黄色葡萄球菌膜结构,导致碱性磷酸酶和DNA泄漏,从而导致细菌死亡。化合物6可通过破坏细胞壁和细胞膜发挥抗菌作用机制。This study aimed to investigate the chemical constituents and antibacterial mechanism of Bletilla striata(Thunb.)Reichb.f.against Staphylococcus aureus.A comprehensive chromatographic separation was conducted to isolate and purify active compounds from B.striata,then all the isolated compounds were identified by NMR spectra.The inhibitory effect of the isolated compounds on S.aureus was evaluated via broth microdilution.The antibacterial mechanism of the active ingredients in B.striata was explored from several aspects including the growth curves of S.aureus,extracellular alkaline phosphatase content,DNA leakage,cell wall and cell membrane integrity.Ten compounds were isolated and identified as shancidin(1),coelonin(2),lusianthridin(3),orchinol(4),isoshancidin(5),blestriarene A(6),(E)-3-(4-hydroxyphenyl)acrylic acid(7),3-phenylpropanoic acid(8),ethyl 2-(4-hydroxyphenyl)acetate(9),ethyl 2-(4-ethylphenyl)acetate(10).Besides,compounds 7-10 were isolated from B.striata for the first time.Furthermore,compound 6 exerted an excellent bacterial inhibitory effect with a minimum inhibitory concentration of 10μg/mL(positive control amoxicillin 0.8μg/mL).The antibacterial mechanism results indicated that compound 6 disrupted the bacterial membrane structure,leading to alkaline phosphatase and DNA leakage,which resulted in bacterial death.Compound 6 may exert antibacterial activity by destroying cell wall and cell membrane.

关 键 词：白及 blestriarene A 抗菌活性 膜损伤 

分 类 号：R932[医药卫生—生药学]