海洋真菌Aspergillus jensenii SS5中化学成分研究  

Chemical constituents of marine fungus Aspergillus jensenii SS5

作　　者：胡靖瑶 袁瑞瑛[1,2] 王广明 蔡诗琪 周明琦 王富乾 蔡由生[2] HU Jing-yao;YUAN Rui-ying;WANG Guang-ming;CAI Shi-qi;ZHOU Ming-qi;WANG Fu-qian;CAI You-sheng(Medical College,Tibet University,Lhasa 850012,China;School of Pharmaceutical Sciences,Wuhan University,Wuhan 430071,China;Department of Pharmacy,Wuhan No.1 Hospital,Wuhan 430022,China)

机构地区：[1]西藏大学医学院,拉萨850012 [2]武汉大学药学院,武汉430071 [3]武汉市第一医院药学部,武汉430022

出　　处：《天然产物研究与开发》2023年第11期1902-1906,共5页Natural Product Research and Development

基　　金：科技部重点研发项目(2018YFC0311000);湖北省自然科学基金面上项目(2020CFB726,2021CFB347);武汉市卫健委医学科研项目(WX20A14)。

摘　　要：利用多种柱色谱分离技术从海洋真菌Aspergillus jensenii SS5的液体发酵产物中分离获得4个已知化合物,并通过NMR、HR-ESI-MS、X单晶衍射等技术鉴定了它们的结构,分别是epigriseofulvin(1)、sterigmatocystin(2)、brevianamide M(3)、meleagrin(4),其中化合物1~3是首次从曲霉属真菌A.jensenii中分离获得。采用SRB法对分离获得的天然产物进行体外细胞毒性测试,结果表明化合物4对人肺癌A549细胞和人肝癌Bel-7402细胞均有较好抑制作用,化合物2对A549人肺癌细胞株有较好抑制作用。本研究作为海洋真菌A.jensenii SS5的化学成分的补充研究,发现了2个具有较好细胞毒活性的化合物,为曲霉属海洋真菌活性天然产物的开发提供了理论依据。Four natural products were isolated from the fermentation extract of the marine fungus Aspergillus jensenii SS5 using various column chromatographic separation techniques,and their structures were identified as epigriseofulvin(1),sterigmatocystin(2),brevianamide M(3),and meleagrin(4)by NMR,HR-ESI-MS and X single-crystal diffraction.Compounds 1-3 were obtained for the first time,and all the compound were tested for in vitro cytotoxicity by SRB assay.The results of cytotoxic activity test showed that compound 4 had inhibitory effect on human lung cancer A549 and human liver cancer Bel-7402,and compound 2 had inhibitory effect on A549.The study of the chemical composition of the marine fungus A.jensenii SS5 has some guiding significance,and two compounds were found to have good cytotoxic activity,which provides some theoretical basis for the development of active natural products of marine fungi of the genus Aspergillus.

关 键 词：海洋真菌Aspergillus jensenii SS5 化学成分 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]