肥胖人群外周血嗜酸性粒细胞数量和活性表达  

Number and activity of peripheral blood eosinophils in obese people

作　　者：程序 杨忖卿 庞博[1] 谷春 侯雪筠[1] 费佳欣 吴敏[2] 李军[2] 刘贵建[1] CHENG Xu;YANG Cunqing;PANG Bo;GU Chun;HOU Xueyun;FEI Jiaxin;WU Min;LI Jun;LIU Guijian(Department of Clinical Laboratory,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;Department of Cardiovascular,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]中国中医科学院广安门医院检验科,北京100053 [2]中国中医科学院广安门医院心血管科,北京100053

出　　处：《检验医学》2023年第9期855-859,共5页Laboratory Medicine

基　　金：国家自然科学基金青年基金(81803975)。

摘　　要：目的分析肥胖人群外周血嗜酸性粒细胞数量和活性表达情况。方法选取2021年6—9月中国中医科学院广安门医院>40岁的体检者41名,根据体重指数(BMI)分为肥胖组(20例,BMI≥30 kg/m2)和对照组(21例,18.5kg/m2<BMI<28kg/m2)。收集所有研究对象一般资料。采用流式细胞术检测外周血嗜酸性粒细胞CD62L、CD63表达情况;采用酶联免疫吸附试验(ELISA)检测血清嗜酸性粒细胞阳离子蛋白(ECP)水平。比较2个组外周血嗜酸性粒细胞数量、百分比和CD62L、CD63、ECP表达的差异。结果肥胖组外周血嗜酸性粒细胞、中性粒细胞数量和百分比、血清ECP水平均稍高于对照组,但差异无统计学意义(P>0.05);CD62L水平低于对照组(P<0.05),CD63高于对照组(P<0.01)。CD62L与BMI呈显著负相关(r=-0.332,P=0.034)。结论肥胖可能与外周血嗜酸性粒细胞活性显著增强有关。Objective To analyze the number and activity of peripheral blood eosinophils in obese people.Methods A total of 41 subjects>40 years old at Guang'anmen Hospital from June to September 2021 were enrolled.According to body mass index(BMI),the subjects were classified into obese group(20 cases,BMI≥30 kg/m2)and control group(21 cases,18.5 kg/m2<BMI<28 kg/m2).The general information were collected.The expressions of CD62L and CD63 in peripheral blood eosinophils were determined by flow cytometry.The level of serum eosinophil cationic protein(ECP)was determined by enzyme-linked immunosorbent assay(ELISA).The number and percentage of peripheral blood eosinophils and the expressions of CD62L,CD63 and ECP were compared between the 2 groups.Results The number and percentage of peripheral blood eosinophils and neutrophils and serum ECP levels in obese group were higher than those in control group(P>0.05).CD62L was lower than that in control group(P<0.05),and CD63 was higher than that in control group(P<0.01).CD62L was negatively correlated with BMI(r=-0.332,P=0.034).Conclusions Obesity might be related to the increase of peripheral blood eosinophil activity.

关 键 词：嗜酸性粒细胞 嗜酸性粒细胞阳离子蛋白 肥胖 

分 类 号：R446.11[医药卫生—诊断学]