XppI调控水稻白叶枯病菌致病力的潜在机制  

A putative mechanism underlining regulation of the virulence of Xanthomonas oryzae pv.oryzae by XppI

作　　者：于燕燕 夏影影 吴可建 占昭宏 陶均[1] YU Yanyan;XIA Yingying;WU Kejian;ZHAN Zhaohong;TAO Jun(Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Tropical Crops,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学热带作物学院/海南省热带生物资源可持续利用重点实验室,海口570228

出　　处：《热带生物学报》2023年第6期642-650,共9页Journal of Tropical Biology

基　　金：国家自然科学基金项目(31960567)。

摘　　要：为了解肽基脯氨酰顺反异构酶XppI(Xanthomonas peptidyl-prolyl isomerase)在Xoo(Xanthomonas oryzae pv.oryzae)与水稻互作中的作用,首先通过体外酶活实验分析了XppI的异构酶活性,随后通过同源重组法构建了xppI的突变体及其互补菌株,并分析了XppI对病原菌生长、致病力、胞外酶活性及抗氧化胁迫能力的调控作用。结果显示:XppI具有肽基脯氨酰顺反异构酶活性,能够正调控Xoo的致病力和抗氧化胁迫的能力,但不影响病原菌的生长和胞外酶活性。以上结果表明,XppI可能通过调控与抗氧化相关途径蛋白的构型影响病原菌应对宿主氧胁迫的能力,进而增强Xoo的致病力。In order to uncover the roles of Xanthomonas peptidyl-prolyl isomerase(XppI)in the interaction between Xanthomonas oryzae pv.oryzae(Xoo)and rice,we detected the isomerase activity of XppI in vitro,constructed the mutant and complementary strains of XppI by homologous recombination method,and analyzed the roles of XppI in growth,pathogenicity,extracellular enzyme activity and antioxidant ability of Xoo.The results showed that XppI had peptidyl prolyl cis-trans isomerase activity,and positively regulated the virulence and antioxidant ability of Xoo,but did not affect the growth and extracellular enzyme activities,which indicates that XppI may affect the ability of the causal agent Xoo to cope with oxygen stress in the host by regulating the conformation of antioxidation-related pathway proteins,thus enhancing the Xoo virulence.

关 键 词：稻属黄单胞菌 肽基脯氨酰顺反异构酶 致病力 抗氧化胁迫 

分 类 号：S432.4[农业科学—植物病理学]