斑翅果蝇解毒代谢相关基因的鉴定与分析  

Identification and analysis of detoxification genes in Drosophila suzukii Matsumura

作　　者：王正磊 刘凯扬 袁琳琳 李芬[1,2] 吴少英 WANG Zhenglei;LIU Kaiyang;YUAN Linlin;LI Fen;WU Shaoying(Sanya Nanfan Research Institute,Hainan University,Haikou,Hainan 572025,China;School of Plant Protection,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学三亚南繁研究院,海南三亚572025 [2]海南大学植物保护学院,海口570228

出　　处：《热带生物学报》2023年第6期660-667,共8页Journal of Tropical Biology

基　　金：国家自然科学基金(31960539);国家重点研发计划项目(2021YFC2600600);海南省重大科技计划项目(ZDKJ2021016)。

摘　　要：根据斑翅果蝇(Drosophila suzukii Matsumura)的转录组数据,通过序列分析、进化分析、结构域及保守氨基酸分析,鉴定出5种斑翅果蝇的解毒代谢基因,包括ABC转运蛋白(ATP-binding cassette transporter,ABCs)、细胞色素P450单加氧酶(cytochrome P450,CYPs)、谷胱甘肽-S-转移酶(glutathione S-transferases,GSTs)、羧酸酯酶(carboxylesterases,CarEs)、尿苷二磷酸葡萄糖醛酸转移酶(UDP-glucuronosyl transferases,UGTs)。进化分析结果显示,斑翅果蝇的CYPs基因分布于CYP3、CYP4、CYP6、CYP9四个进化分支,ABCs基因分布于ABCC、ABCD、ABCF、ABCG四个亚家族,GSTs基因分布于GST-Delta、GST-Epsilon、GST-Theta以及GST-Zeta四个进化分支,斑翅果蝇的CarEs基因和UGTs基因与亚艳丽果蝇(Drosophila subpulchrella)的遗传距离最近;结构域及保守氨基酸分析结果表明所有的CYPs均包含P450结构域;ABCs中都包含ABC2结构域;在GSTs中都含有GST结构域;在CarEs中包含Coesterases结构域;在UGTs中含有UDPGT结构域。CYPs的保守基序为EEGGKKRNDFLDLLJZLKKEG,ABCs的保守基序为DCPSASNPADYIIE,GSTs的保守基序为LYPKDLVKRAVVDQRLHFE。Drosophila suzukii Matsumura is a worldwide fruit pest.Based on the transcriptome of D.suzukii,five metabolic detoxification genes were identified by using sequence analysis,phylogenetic analysis,structural domain analysis and conserved motif analyses.These five genes include ATP-binding cassette transporter(ABCs),cytochrome P450(CYPs),glutathione S-transferases(GSTs),carboxylesterases(CarEs)and UDPglucuronosyl-transferases(UGTs).Phylogenetic analysis showed that CYPs genes were classified into four clans,CYP3,CYP4,CYP6 and CYP9,while ABCs genes fell into four clans,ABCC,ABCD,ABCF and ABCG.GSTs genes were classified into GST-Delta,GST-Epsilon,GST-Theta and GST-Zeta.The CarEs genes and UGTs genes of D.suzukii were the closest to those of Drosophila subpulchrella.Structural domain and conserved motif analyses showed that CYPs had a conserved P450 domain;ABCs had a conserved ABC2 domain;GSTs had a conserved GST domain;CarEs had a conserved Coestrases domain;UGTs had a conserved UDPGT domain.Conservative motifs of CYPs were EEGGKKRNDFLDLLJZLKKEG.Conservative motifs of ABCs were DCPSASNPADYIIE.Conservative motifs of GSTs were LYPKDLVKRAVVDQRLHFE.

关 键 词：斑翅果蝇 代谢解毒基因 进化分析 蛋白结构域 氨基酸残基 

分 类 号：Q966[生物学—昆虫学]