应用于植物生理状态检测的低成本叶绿素荧光成像系统  

Low-Cost Chlorophyll Fluorescence Imaging System Applied in Plant Physiology Status Detection

作　　者：杨振宇 汤浩 葛玮 夏倩 仝德之 付丽疆 郭亚 YANG Zhenyu;TANG Hao;GE Wei;XIA Qian;TONG Dezhi;FU Lijiang;GUO Ya(Key Laboratory of Advanced Process Control for Light Industry,Ministry of Education,Jiangnan University,Wuxi 214122,China;School of Internet of Things,Jiangnan University,Wuxi 214122,China;Chloview Science and Technology(Wuxi)Co.Ltd,Wuxi 214000,China)

机构地区：[1]江南大学轻工过程先进控制教育部重点实验室,江苏无锡214122 [2]江南大学物联网工程学院,江苏无锡214026 [3]绿视芯科技(无锡)有限公司,江苏无锡214000

出　　处：《智慧农业（中英文）》2023年第3期154-165,共12页Smart Agriculture

基　　金：国家自然科学基金国际合作项目(51961125102);国家自然科学基金面上项目(31771680);江苏省农业科技自主创新资金项目(SCX(22)3669)。

摘　　要：[目的/意义]植物光合作用过程中释放的叶绿素荧光与光化学反应紧密耦合,其荧光信号采集是光合作用效率、植物生理及环境胁迫等无损的测试手段。作为获取该信号的叶绿素荧光成像系统通常价格昂贵,针对此问题,本文提出一种低成本叶绿素荧光成像系统设计方案。[方法]叶绿素荧光成像系统主要由激发光灯组、CMOS(Complementary Metal Oxide Semiconductor)相机及其控制电路和智能手机上位机三部分组成。激光发灯组采用LED面光源和碗状结构,通过对光场的仿真分析保证光照强度和均匀性;采用微型CMOS相机进行荧光图像采集,利用智能手机作为上位机完成激发光控制,并将数据回传至手机或服务器进行分析、处理、存储及显示。[结果和讨论]基于该方案,制造了一款仪器样机,其激发光强最大为6250μmol/(m~2·s),光场整体变化幅度偏差为2%,光谱范围为400~1000 nm,稳定的采集频率最高可达42 f/s,具备连续光激发和调制脉冲激发功能。[结论]通过叶绿素荧光图像采集实验验证了本仪器的有效性。该仪器结构简单、造价成本低,在植物生理状态检测领域有着很好的应用价值。[Objective]Chlorophyll fluorescence(ChlF)emission from photosystem II(PSII)is closely coupled with photochemical reactions.As an efficient and non-destructive means of obtaining plant photosynthesis efficiency and physiological state information,the collec‐tion of fluorescence signals is often used in many fields such as plant physiological research,smart agricultural information sensing,etc.Chlorophyll fluorescence imaging systems,which is the experimental device for collecting the fluorescence signal,have difficul‐ties in application due to their high price and complex structure.In order to solve the issues,this paper investigates and constructs a low-cost chlorophyll fluorescence imaging system based on a micro complementary metal oxide semiconductor(CMOS)camera and a smartphone,and carries out experimental verifications and applications on it.[Method]The chlorophyll fluorescence imaging system is mainly composed of three parts:excitation light,CMOS camera and its control circuit,and a upper computer based on a smartphone.The light source of the excitation light group is based on the principle and characteristics of chlorophyll fluorescence,and uses a blue light source of 460 nm band to achieve the best fluorescence excitation effect.In terms of structure,the principle of integrating sphere was borrowed,the bowl-shaped light source structure was adopted,and the design of the LED surface light source was used to meet the requirements of chlorophyll fluorescence signal measurement for the uniformity of the excitation light field.For the adjustment of light source intensity,the control scheme of pulse width modulation was adopted,which could realize sequential control of different intensities of excitation light.Through the simulation analysis of the light field,the light intensity and distribution characteristics of the light field were stuidied,and the calibration of the excitation light group was completed according to the simulation results.The OV5640 micro CMOS camera was used to collect fluorescence ima

关 键 词：荧光检测 叶绿素荧光成像系统 光合作用 CMOS相机 TRACEPRO 

分 类 号：TP23[自动化与计算机技术—检测技术与自动化装置] Q-334[自动化与计算机技术—控制科学与工程]