Altered microRNA expression profiles of human spermatozoa in normal fertile men of different ages  

作　　者：Ming-Jia Zhao Yao-Nan Zhang Yong-Ping Zhao Xian-Bing Chen Bao-Sheng Han Ning Ding Yi-Qun Gu Shu-Song Wang Jing Ma Mei-Ling Liu 

机构地区：[1]Graduate School of Peking Union Medical College,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100730,China [2]National Health Commission Key Laboratory of Male Reproductive Health,National Research Institute for Family Planning,Beijing 100081,China [3]Department of Reproduction and Genetics,Maternity and Child Health Care Hospital,Tangshan 063000,China [4]Department of Family Planning and Reproductive Medicine,Peking University People’s Hospital,Beijing 100081,China [5]Hebei Key Laboratory of Reproductive Medicine,Hebei Institute of Reproductive Health Science and Technology,Shijiazhuang 050071,China

出　　处：《Asian Journal of Andrology》2023年第6期737-744,共8页亚洲男性学杂志（英文版）

基　　金：This work was supported by the Central Public Interest Scientific Institution Basal Research Fund of National Research Institute for Family Planning(No.2021GJZ09);the CAMS Innovation Fund for Medical Sciences(CIFMS,No.2018-I2M-1-004)。

摘　　要：MicroRNAs(miRNAs)are mediators of the aging process.The purpose of this work was to analyze the miRNA expression profiles of spermatozoa from men of different ages with normal fertility.Twenty-seven donors were divided into three groups by age(Group A,n=8,age:20–30 years;Group B,n=10,age:31–40 years;and Group C,n=9,age:41–55 years)for high-throughput sequencing analysis.Samples from 65 individuals(22,22,and 21 in Groups A,B,and C,respectively)were used for validation by quantitative real-time polymerase chain reaction(qRT-PCR).A total of 2160 miRNAs were detected:1223 were known,937 were newly discovered and unnamed,of which 191 were expressed in all donors.A total of 7,5,and 17 differentially expressed microRNAs(DEMs)were found in Group A vs B,Group B vs C,and Group A vs C comparisons,respectively.Twenty-two miRNAs were statistically correlated with age.Twelve miRNAs were identified as age-associated miRNAs,including hsa-miR-127-3p,mmu-miR-5100_L+2R-1,efu-miR-9226_L-2_1ss22GA,cgr-miR-1260_L+1,hsa-miR-652-3p_R+1,pal-miR-9993a-3p_L+2R-1,hsa-miR-7977_1ss6AG,hsa-miR-106b-3p_R-1,hsa-miR-186-5p,PC-3p-59611_111,hsa-miR-93-3p_R+1,and aeca-mir-8986ap5_1ss1GA.There were 9165 target genes of age-associated miRNAs.Gene Ontology(GO)analysis of the target genes identified revealed enrichment of protein binding,membrane,cell cycle,and so on.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis of age-related miRNAs for target genes revealed 139 enriched pathways,such as signaling pathways regulating stem cell pluripotency,metabolic pathways,and the Hippo signaling pathway.This suggests that miRNAs play a key role in male fertility changes with increasing age and provides new evidence for the study of the mechanism of age-related male fertility decline.

关 键 词：age FERTILITY MICRORNA SPERMATOZOA 

分 类 号：R697[医药卫生—泌尿科学]