NG2细胞的增殖活化对大鼠脊髓损伤后神经病理性疼痛产生和维持的影响  

NG2 cell proliferation and activation generate and maintain neuropathic pain in rats after spinal cord injury

作　　者：陈业煌 魏梁锋 陈恺钦 郑兆聪 黄预备 吴箭午 薛亮 王守森 Chen Yehuang;Wei Liangfeng;Chen Kaiqin;Zheng Zhaocong;Huang Yubei;Wu Jianwu;Xue Liang;Wang Shousen(Department of Neurosurgery,Fuzong Clinical Medical College of Fujian Medical University(The 900^(th)Hospital of Joint Logistic Team of PLA),Fuzhou 350025,China)

机构地区：[1]福建医科大学福总临床医学院(解放军联勤保障部队第九〇〇医院)神经外科,福州350025

出　　处：《中华神经医学杂志》2023年第10期994-1000,共7页Chinese Journal of Neuromedicine

基　　金：福建省自然科学基金(2021J011276);联勤保障部队第九〇〇医院临床应用研究专项课题(2020L18)

摘　　要：目的:探讨NG2细胞在脊髓损伤(SCI)后神经病理性疼痛的产生和维持中的作用。方法:将100只健康成年雄性SD大鼠按随机数字表法分为对照组( n=20,不做任何干预措施)、假手术组( n=40,暴露T 10节段但不实施脊髓撞击)及SCI组( n=40,暴露T 10节段并采用改进型Allen's法构建SCI模型)。分别于术前1 d及术后14 d、21 d、28 d时,应用Von Frey纤维探针检测大鼠后肢的机械缩足反射阈值(MWT),采用免疫荧光染色检测大鼠脊髓背角NG2阳性细胞数量占细胞总数比例,应用Western blotting实验检测大鼠脊髓背角硫酸软骨素蛋白多糖(CSPG)的表达。 结果:术后14 d、21 d、28 d时,SCI组大鼠后肢MWT均明显低于对照组及假手术组,脊髓背角NG2阳性细胞数量占细胞总数比例均明显高于对照组及假手术组,脊髓背角CSPG表达均明显高于对照组及假手术组,差异均有统计学意义( P<0.05)。SCI组大鼠术前1 d及术后14 d、21 d、28 d时的后肢MWT呈现先下降后回升趋势,脊髓背角NG2阳性细胞数量占细胞总数比例呈现先上升后回降趋势,脊髓背角CSPG表达呈现先上升后回降趋势;除术后21 d与术后28 d间的差异无统计学意义( P>0.05)外,其余各时间点间后肢MWT、脊髓背角NG2阳性细胞数量占细胞总数比例及脊髓背角CSPG表达的差异均有统计学意义( P<0.05)。SCI组大鼠后肢MWT与脊髓背角NG2阳性细胞数量占细胞总数比例呈负相关关系( r=-0.876, P<0.001),脊髓背角CSPG表达与NG2阳性细胞数量占细胞总数比例呈正相关关系( r=0.927, P<0.001)。 结论:SCI后脊髓组织中NG2细胞的增殖及其分泌的CSPG表达增加参与了神经病理性疼痛的产生和维持。Objective To investigate the role of NG2 cells in generating and maintaining neuropathic pain in rats after spinal cord injury(SCI).Methods According to random number table method,100 healthy adult male SD rats were divided into control group(n=20,without any intervention),sham-operated group(n=40,exposed T10 segment without spinal cord impact)and SCI group(n=40,exposed T10 segment and constructed SCI model by improved Allen's method).One d before,and 14,21 and 28 d after surgery,Von Frey fiber probe was used to detect the rat hindlimb mechanical withdrawal threshold(MWT);immunofluorescent staining was used to detect the proportion of NG2-positive cells in spinal dorsal horn cells;Western blotting was used to detect chondroitin sulfate proteoglycan(CSPG)expression in spinal dorsal horn of rats.Results Fourteen,21 and 28 d after surgery,SCI group had significantly lower hindlimb MWT,and significantly higher proportion of NG2-positive cells in spinal dorsal horn cells and CSPG expression in spinal dorsal horn than control group and sham-operated group(P<0.05).One d before,and 14,21 and 28 d after surgery,in SCI group,hindlimb MWT decreased firstly and increased secondly,proportion of NG2-positive cells in spinal dorsal horn cells increased firstly and decreased secondly,and CSPG expression in spinal dorsal horn increased firstly and decreased secondly.Except for those 21 and 28 d after surgery,hindlimb MWT,proportion of NG2-positive cells in spinal dorsal horn cells,and CSPG expression in spinal dorsal horn showed significant differences between each two time points(P<0.05).In SCI group,hindlimb MWT was negatively correlated with proportion of NG2-positive cells in spinal dorsal horn cells(r=-0.876,P<0.001),and CSPG expression was positively correlated with proportion of NG2-positive cells in spinal dorsal horn cells(r=0.927,P<0.001).Conclusion NG2 cell proliferation and increased CSPG expression secreted by NG2 cells in spinal cord tissues after SCI generate and maintain neuropathic pain.

关 键 词：脊髓损伤 神经病理性疼痛 NG2细胞 硫酸软骨素蛋白多糖 

分 类 号：R651.2[医药卫生—外科学]