复发性流产患者胎盘绒毛中FTO mRNA和ALKBH5 mRNA水平表达及临床价值研究  

Study on the Expression and Clinical Value of FTO mRNA and ALKBH5 mRNA Levels in Placental Villi of Patients with Recurrent Miscarriage

作　　者：陈静 邓健美 周瑞娴 黄真轩 郑晓芳 CHEN Jing;DENG Jianmei;ZHOU Ruixian;HUANG Zhenxuan;ZHENG Xiaofang(Department of Obstetrics,Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine,Guangdong Shenzhen 518104,China)

机构地区：[1]深圳市中西医结合医院产科,广东深圳518104

出　　处：《现代检验医学杂志》2023年第6期71-74,97,共5页Journal of Modern Laboratory Medicine

摘　　要：目的 探讨胎盘绒毛组织中肥胖相关蛋白(fat mass and obesity associated protein,FTO)mRNA和AlkB同源蛋白5(Alk B homologous 5,ALKBH5)mRNA水平与复发性流产的相关性。方法 选取2019年12月~2021年1月深圳市中西医结合医院产科接收的98例复发性流产患者为复发性流产组,同期选取正常早孕人工流产者96例作为对照组。比较两组一般资料;采用实时荧光定量PCR(qRT-PCR)法检测两组受试者胎盘绒毛组织FTO mRNA和ALKBH5mRNA水平;采用Pearson法分析复发性流产患者胎盘绒毛组织FTO mRNA与ALKBH5 mRNA水平相关性;采用单因素及多因素Logistic回归分析影响复发性流产的因素。结果 复发性流产组胎盘绒毛组织FTO mRNA(0.72±0.16),ALKBH5 mRNA(0.64±0.18)水平低于对照组(1.02±0.24,1.01±0.21),差异均有统计学意义(t=10.263,13.185,均P <0.05)。复发性流产患者胎盘绒毛组织FTO mRNA与ALKBH5 mRNA水平呈正相关(r=0.537,P <0.05)。流产次数> 3次的复发性流产患者胎盘绒毛组织FTO mRNA(0.40±0.12),ALKBH5 mRNA(0.47±0.11)水平低于流产次数3次患者(0.86±0.18,0.72±0.14),差异具有统计学意义(t=12.615,8.561,均P <0.05)。单因素Logistic回归分析结果显示,孕囊大小为复发性流产发生的危险因素[OR(95%CI):2.025(1.468~2.794),P <0.05],FTO mRNA,ALKBH5 mRNA为复发性流产发生的保护因素[OR(95%CI):0.730(0.548~0.972);0.655(0.492~0.873),均P <0.05]。多因素Logistic回归分析结果显示,FTO mRNA和ALKBH5 mRNA为复发性流产发生的独立保护因素[OR(95%CI):0.674(0.519~0.874),0.541(0.392~0.747),均P <0.05]。结论 复发性流产患者胎盘绒毛组织FTO mRNA和ALKBH5 mRNA低表达,二者为复发性流产发生的独立保护因素。Objective To investigate the correlation between the levels of fat mass and obesity associated protein(FTO)mRNA and AlkB homologues 5(ALKBH5)mRNA in placental villi tissues and recurrent miscarriage.Methods From December 2019 to January 2021,98 patients with recurrent miscarriage received in Obstetrics Department of Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine were selected as the research objects(recurrent miscarriage group),and 96 patients with normal early pregnancy induced abortion were selected as control(control group)during the same period.The general data of the two groups were compared;the levels of FTO mRNA and ALKBH5 mRNA in the placental villi tissues of the two groups of subjects were detected by real-time fluorescent quantitative PCR(qRT-PCR).The correlation between FTO mRNA and ALKBH5 mRNA levels in the placental villi of patients with recurrent miscarriage was detected by Pearson method.The factors affecting recurrent miscarriage were detected by single factor and multivariate Logistic regression.Results The levels of FTO mRNA(0.72±0.16)and ALKBH5 mRNA(0.64±0.18)in placental villi in the recurrent miscarriage group were lower than those in the control group(1.02±0.24,1.01±0.21),and the differences were statistically significant(t=10.263,13.185,all P<0.05).There was a positive correlation between FTO mRNA and ALKBH5 mRNA levels in the placental villi of patients with recurrent miscarriage(r=0.537,P<0.05).The levels of FTO mRNA(0.40±0.12)and ALKBH5 mRNA(0.47±0.11)in the placental villous tissue of patients with recurrent miscarriage more than 3 miscarriages were lower than those of patients with 3 miscarriages(0.86±0.18,0.72±0.14),and the differences were statistically significant(t=12.615,8.561,all P<0.05).The results of univariate logistic regression analysis showed that the gestational sac size was a risk factor affecting the occurrence of recurrent miscarriage[OR(95%CI):2.025(1.468~2.794),P<0.05],and FTO mRNA and ALKBH5 mRNA were protective factors affecting

关 键 词：复发性流产 胎盘 绒毛组织 肥胖相关蛋白mRNA AlkB同源蛋白5 mRNA 

分 类 号：R714.21[医药卫生—妇产科学] R392.11[医药卫生—临床医学]