琅琊鸡FSHR基因-868位点的多态性及对产蛋性能的遗传效应研究  

Study on the Polymorphism and Genetic Effect of the-868 Locus of FSHR Gene on Egg Laying Traits in Langya Chickens

作　　者：陈诚[1] 乔西波 孙亿[1] 康丽[1] 姜运良[1] CHEN Cheng;QIAO Xibo;SUN Yi;KANG Li;JIANG Yunliang(Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,Shandong Agricultural University,Tai’an 271018,China;Shandong Jihua Poultry Breeding Co.Ltd.,Juxian 276800,China)

机构地区：[1]山东农业大学、山东省动物生物工程与疾病防治重点实验室,泰安271018 [2]山东纪华家禽育种股份有限公司,莒县276800

出　　处：《畜牧兽医学报》2023年第11期4560-4568,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划(2021YFD1300100);山东省农业良种工程(2019LZGC019)。

摘　　要：旨在探讨琅琊鸡FSHR基因-868位点的多态性和遗传效应。本研究采用PCR扩增和琼脂糖凝胶电泳对相同饲养管理条件下的、有产蛋性能记录的382只琅琊鸡母鸡FSHR 5′调控区-868位点进行了基因型判定并分析了群体的多态性,分析了该位点与产蛋性状的关系;采用qRT-PCR和Western Blotting对不同基因型的琅琊鸡个体FSHR的表达量进行了比较。结果表明:琅琊鸡FSHR 5′调控区-868位点存在多态性,I-为优势等位基因,群体偏离哈代-温伯格平衡状态(P<0.05);I+I-基因型个体的47周产蛋数(E47)和换羽后134 d的产蛋数(134D)均显著高于I-I-基因型个体(P<0.05),I+I+和I+I-基因型个体最大连产天数(MCS)显著高于I-I-基因型个体(P<0.01);在等级前卵泡中,I+I+基因型个体的FSHR mRNA水平显著高于I-I-基因型个体(P<0.05);在鸡卵巢中,I+I-基因型个体的FSHR蛋白水平显著高于I-I-基因型个体(P<0.05)。琅琊鸡FSHR基因-868位点存在多态性,FSHR 5′调控区-868位点多态性对琅琊鸡E47和MCS效应显著,亦对换羽后134 d的产蛋量产生显著影响,I+I+基因型个体的FSHR表达量较高。通过基因型选择逐步降低I-的等位基因频率,预期可以提高琅琊鸡的产蛋性能。The study aimed to analyze the polymorphism and genetic effects of-868 locus of FSHR gene in Langya chickens.Genotyping of the-868 locus of FSHR gene were performed by PCR amplification and electrophoresis on 382 Langya hens that were raised with the same feed management and were recorded individually for egg performance,and the correlation between the locus and egg laying traits was analyzed.Quantitative real-time PCR(qRT-PCR)and Western blotting were used to compare mRNA and protein expression levels of chicken FSHR.The results showed that,the polymorphism of-868 locus in FSHR 5′regulatory region in the Langya chicken population was found,I-was the dominant allele,and this population deviated from the Hardy-Weinberg equilibrium(P<0.05).The egg number at 47 weeks(E47)and the egg number at 134 days after molting(134D)were both significantly higher for hens with I+I+than those with I-I-(P<0.05).The maximum clutch size(MCS)was significantly higher for hens with I+I+and I+I-than those with I-I-(P<0.01).The expression of FSHR mRNA in pre-hierarchical follicles was significantly higher for hens with genotype I+I+than those with I-I-(P<0.05).The expression of FSHR protein in the ovary was significantly higher for hens with genotype I+I-than those with I-I-(P<0.05).There were polymorphism at the-868 locus of FSHR gene in Langya chickens,the genetic effect of genotypes on E47,MCS and 134D was significant and hens with genotype I+I+exhibited higher expression level of FSHR.In Langya chicken breeding,egg laying performance is expected to increase by decreasing the allele frequency of I-.

关 键 词：琅琊鸡 FSHR -868位点多态性 产蛋性状 表达量 

分 类 号：S831.2[农业科学—畜牧学]