紫锥菊提取物联合柳氮磺吡啶对湿热泄泻大鼠Th17/Treg免疫失衡的影响  

Effect of Echinacea purpurea Extract Combined with Sulfasalazine on Immune Imbalance of Th17/Treg in Rats with Damp-Heat Syndrome

作　　者：池幸子 李耀星 王慧婷 严铭恩 杨诗靖 杨泊文 孙晗 郭世宁[1,2] 石达友 武力[1,2] 刘翠 CHI Xingzi;LI Yaoxing;WANG Huiting;YAN Ming’en;YANG Shijing;YANG Bowen;SUN Han;GUO Shining;SHI Dayou;WU Li;LIU Cui(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Nature Medicine,Guangzhou 510642,China)

机构地区：[1]华南农业大学兽医学院,广州510642 [2]广东省兽用中药与天然药物工程技术研究中心,广州510642

出　　处：《畜牧兽医学报》2023年第11期4817-4826,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(32273046);广东省基础与应用基础研究基金(2022A1515011692)。

摘　　要：本研究采用紫锥菊提取物与柳氮磺吡啶治疗湿热泄泻大鼠,探讨中西药物结合对大鼠湿热泄泻的疗效,为湿热泄泻疾病的治疗提供科学依据。大鼠随机分为5组,除空白组外,其余大鼠采用“内外湿热+大肠杆菌”建立湿热泄泻大鼠模型,随后紫锥菊提取物组、柳氮黄吡啶组和中西结合组大鼠分别给予1 g·kg^(-1)紫锥菊提取物、0.1 g·kg^(-1)柳氮磺吡啶、1 g·kg^(-1)紫锥菊提取物+0.1 g·kg^(-1)柳氮磺吡啶,采用临床症状评分评价疾病模型,测定各组大鼠血清炎性因子及外周血单核细胞(PBMCs)中Th17/Treg细胞比例。结果表明,造模后大鼠精神不佳,饮食量下降,运动量减少,被毛粗糙泛黄,排出黄褐色软便。给药6 d后,紫锥菊提取物组、柳氮磺吡啶组与中西结合组大鼠精神、饮食及临床表现均恢复正常。与空白组相比,模型组大鼠血清中IL-17、IL-23水平显著升高(P<0.05),血清中TGF-β、IL-10水平显著降低(P<0.05);PBMCs中Th17细胞比例显著升高(P<0.05),Treg细胞比例显著降低(P<0.05),Th17/Treg比例显著升高(P<0.05)。与模型组相比,紫锥菊提取物组与中西结合组大鼠血清中IL-17、IL-23水平显著降低(P<0.05),血清中TGF-β水平显著升高(P<0.05);大鼠PBMCs中Th17细胞比例显著降低(P<0.05),Treg细胞比例显著升高(P<0.05),Th17/Treg比例显著降低(P<0.05)。综上,紫锥菊提取物结合柳氮磺吡啶通过对相关细胞因子的调控降低湿热泄泻模型大鼠失衡的Th17/Treg比例,从而缓解大鼠湿热泄泻。In this study,Echinacea purpurea extract and sulfasalazine were used to treat intestine damp-heat syndrome rats,to explore the efficacy of integrated traditional Chinese and Western Medicine on Intestine Damp-heat syndrome rats,and to provide scientific basis for the treatment of Intestine Damp-heat syndrome diseases.The rats were randomly divided into 5 groups.Except the control group,the rest of the rats were treated with"internal and external Damp-heat+Escherichia coli"to establish Damp-heat syndrome rat model.Then Echinacea purpurea extract group,sulfasalazine group and integrated traditional Chinese and Western medicine group were given 1 g·kg^(-1) Echinacea purpurea extract,0.1 g·kg^(-1) sulfasalazine,1 g·kg^(-1) Echinacea purpurea extract and 0.1 g·kg^(-1) sulfasalazine,respectively.The disease model was evaluated by clinical symptom score.The serum inflammatory factors and the proportion of Th17/Treg cells in PBMCs were measured.The results showed that after modeling,the rats had poor spirit,decreased dietary intake,reduced amount of exercise,rough and yellowish hair,and excreted yellowish brown soft stool.After 6 days of administration,the spirit,dietary intake and appearance of rats in Echinacea purpurea extract group,sulfasalazine group and integrated traditional Chinese and Western medicine group returned to normal.Compared with the control group,the levels of IL-17 and IL-23 in serum in the model group were significantly higher(P<0.05),and the level of TGF-β,IL-10 in serum decreased significantly(P<0.05).In PBMCs of model group,the proportion of Th17 cells increased significantly(P<0.05),the proportion of Treg cells decreased significantly(P<0.05),and the proportion of Th17/Treg increased significantly(P<0.05)compared with the control group.Compared with the model group,the levels of IL-17 and IL-23 in serum in Echinacea purpurea extract group and integrated traditional Chinese and Western medicine group were significantly lower(P<0.05),and the levels of TGF-βin serum increased significantly(P<

关 键 词：紫锥菊提取物 湿热泄泻 TH17/TREG 

分 类 号：S853.74[农业科学—临床兽医学]