一株H1N1亚型猪流感病毒全基因组特征分析  

Genomic Characterization Analysis of a H1N1 Subtype Swine Influenza Virus

作　　者：张傲 谭斌[1] 刘可欣 刘佳利 张淑琴[1] ZHANG Ao;TAN Bin;LIU Kexin;LIU Jiali;ZHANG Shuqin(Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《畜牧兽医学报》2023年第11期4866-4871,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：吉林省重点研发项目(20220202058NC);中国农业科学院科技创新工程项目(CAAS-ASTIP-ISAPS-2023)。

摘　　要：旨在了解吉林地区猪流感病毒(swine influenza virus,SIV)的流行情况并探究其分子生物学特征,本研究采集吉林某猪场疑似SIV感染猪的鼻拭子样品接种鸡胚分离病毒并对该分离株进行全基因组扩增、遗传进化分析、功能性氨基酸位点分析及选择压力分析。结果显示:病毒分离鉴定表明,分离株为H1N1亚型SIV,遗传进化分析显示,分离株全基因组的8个基因节段均属于经典猪流感病毒谱系,未出现流感病毒不同基因型之间的基因重排。功能性氨基酸位点分析显示,毒株出现SIV致病性、毒力及复制能力增强的相关位点突变,NA基因具有神经氨酸酶抑制剂耐药性相关位点突变。选择压力分析显示,SIV HA和NA基因在吉林省的自主变异进化明显,NA的突变率高于HA。本研究获得的H1N1亚型SIV分离株全基因组序列分析结果对SIV流行病学调查及后续疫苗研发具有一定意义。To investigate the prevalence and biological characteristics of swine influenza virus(SIV)in Jilin,the nasal swab samples were collected from swine farm in Jilin and inoculated into SPF chicken embryos for virus isolation.The isolated virus was identified using hemagglutination test and RT-PCR.Whole genome amplification,genetic evolutionary analysis,analysis of functional amino acid mutations,and selection pressure analysis were performed on the isolated strain.RT-PCR analysis confirmed that the isolated strain belonged to the H1N1 subtype of SIV.Genetic evolutionary analysis revealed that all eight gene segments of the strain belonged to the classical lineage of SIV H1N1.No gene rearrangements among different genotypes of influenza viruses were observed.Analysis of functional amino acid sites indicated that the strain had mutations at sites associated with increased pathogenicity,virulence,and replicability of SIV.Mutations in the NA gene were found at sites associated with resistance to neuraminidase inhibitors.Moreover,the HA and NA genes of SIV in Jilin province displayed evidence of autonomous mutations,with higher mutation rates observed in the NA gene compared to the HA gene.Whole genome sequence analysis of the H1N1 subtype SIV isolates in this study provides valuable insights for epidemiological investigations of SIV and future vaccine development.

关 键 词：猪流感病毒 H1N1亚型 分离鉴定 遗传进化分析 选择压力分析 

分 类 号：S852.659.5[农业科学—基础兽医学]