用DNA宏条形码技术分析鹄沼枝额虫的食性  

Analysis of Feeding Habits of Fairy Shrimp Branchinella kugenumaensis Based on DNA Matebarcoding

作　　者：陈江[1] 吴俣学 王艳艳 姚俊杰[1] 何浩宇 CHEN Jiang;WU Yuxue;WANG Yanyan;YAO Junjie;HE Haoyu(Research Center of Fishery Resources and Environment,Guizhou University,Guiyang 550025,China;Guizhou Fisheries Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550025,China)

机构地区：[1]贵州大学渔业资源与环境研究中心,贵州贵阳550025 [2]贵州省农业科学院水产研究所,贵州贵阳550025

出　　处：《水产学杂志》2023年第5期27-31,41,共6页Chinese Journal of Fisheries

基　　金：大水面鲢鳙鱼养殖技术标准制订(黔水合审[2020])。

摘　　要：为了认识野生鹄沼枝额虫(Branchinella kugenumaensis)的食性生物学特性,采样DNA宏条形码技术分析了贵州省都匀市甲登村池塘中鹄沼枝额虫的食性。结果显示:从肠道中共鉴定出151个物种,分属于10个门类,即节肢动物门(Arthropoda)、刺胞动物门(Cnidaria)、脊索动物门(Chordata)、线虫动物门(Nematoda)、软体动物门(Mollusca)、半索动物门(Hemichordata)、环节动物门(Annelida)、扁形动物门(Platyhelminthes)、被子植物门(Angiospermae)和绿藻门(Chlorophyta)。节肢动物门共83种,占食物种类总数的70%,是食物中最丰富的类群;其次是脊索动物门,有27种,占11%;绿藻门占据食物的比例最低,仅为0.3%。结果表明,鹄沼枝额虫主动滤食水体中的有机颗粒,对有机颗粒的滤食无选择性;有机颗粒主要来源于大型溞、蚤状溞、水螅、顶切叶蚁和跳镰猛蚁。In order to understand biological characteristics of the feeding habits of fairy shrimp Branchinella kugenumaensis in wild,the DNA metabarcoding technology was adopted to identify food items in the intestine of the fairy shrimp sampled in ponds in Jiadeng Village,Duyun City,Guizhou Province.The results showed that a total of 151 species were observed in the intestinal tract,belonging to 10 phyla including Arthropoda,Cnidaria,Chordata,Nematoda,Mollusca,Hemichordata,Annelida,Platyhelminthes,Angiospermae,and Chlorophyta,with the most abundant Arthropoda in food,with a total of 83 species,accounting for 70%of the total food items;followed by the species of Chordate,with 27 species,accounting for 11%,and the least Chlorophyta,only 0.3%.It was found that B.kugenumaensis actively filtered organic particles in the water body,without food selectivity.The organic particles were primarily de-rived from water fleas Daphnia magna,Daphnia pulex,Hydra vulgaris,Acromyrmex echinatior and Harpegnathos saltator.

关 键 词：DNA宏条形码技术 鹄沼枝额虫 食性 

分 类 号：S917.4[农业科学—水产科学]