小麦株高主效QTL-qPh-3D遗传效应解析  

Genetic Effects Analysis of qPh-3D,a Major QTL for Plant Height in Common Wheat

作　　者：蔡益彪 孙振仓 史欣瑶 管宇翔 程佳佳 杨爽 王萌鲁 张磊 王晨阳 丁洪科 王发祥 赵春华 孙晗 吴永振 秦冉 崔法 CAI Yibiao;SUN Zhencang;SHI Xinyao;GUAN Yuxiang;CHENG Jiajia;YANG Shuang;WANG Menglu;ZHANG Lei;WANG Chenyang;DING Hongke;WANG Faxiang;ZHAO Chunhua;SUN Han;WU Yongzhen;QIN Ran;CUI Fa(School of Agriculture,Ludong University/Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong,Yantai 264025;Jingbo Agrochemicals Technology Co.,Ltd.,Binzhou 256500,Shandong)

机构地区：[1]鲁东大学农学院/山东省高等学校作物高产抗逆分子模块重点实验室,烟台264025 [2]京博农化科技有限公司,山东滨州256500

出　　处：《植物遗传资源学报》2023年第6期1690-1701,共12页Journal of Plant Genetic Resources

基　　金：山东省重点研发计划(重大创新工程)(2022LZG002-2);国家自然科学基金(32101726,32072051,32272119)。

摘　　要：qPh-3D为1个控制株高的主效QTL,可在科农9204×京411衍生的重组自交系群体(KJ-RILs)的14组环境数据中被稳定检测到,定位于科农9204基因组3D染色体KN3D:515.08~539.08 Mb区间内,其降低株高的等位基因来自京411。本研究继续深入分析该QTL降秆机制,并利用包含187个家系的KJ-RILs群体及316份育成品种(系)组成的自然作图群体,对其进行遗传效应解析,进一步明确其对产量性状的影响。基于KJ-RILs群体分析结果表明,来自京411的qPh-3D降秆基因型通过降低穗部以下各节间长度进而显著降低株高,但对穗长无显著影响。qPh-3D在降低株高的同时,可一定程度降低单株产量。在qPh-3D靶区间内选择2个紧密连锁的标记AX-110160363和AX-111109273,对316份自然作图群体进行产量性状遗传效应和靶区段选择效应分析。结果表明,降秆基因型在降低株高的同时,对穗长具有一定正效应,但对单株产量具有显著负效应。qPh-3D靶区段育种选择效应分析结果表明,北京和陕西对qPh-3D降秆基因型利用率较高;而四川、青海、山东以及国外地区对其利用率较低,且在不同年代中,降秆基因型利用率均较低。随着时间的推移,qPh-3D的增秆基因型利用率呈上升趋势。此外,本研究还开发了1个与qPh-3D紧密连锁的基于PCR技术检测的InDel分子标记。研究结果可对未来qPh-3D分子育种应用提供理论参考。qPh-3D is a major quantitative trait locus(QTL)regulating plant height(PH)in wheat.This locus could be repeatedly identified in datasets at 14 different environments in the recombinant inbred line(RIL)population derived from the cross between Kenong 9204 and Jing 411(denoted as KJ-RILs).qPh-3D was mapped to the chromosomal region of KN3D:515.08-539.08 Mb,and the allele from parent Jing 411 could reduce PH.In this study,we aimed to further characterize the genetic mechanism of PH reduction caused by qPh-3D and clarify its genetic effects on yield-related traits,using the KJ-RILs population containing 187 lines as well as a natural mapping population consisting of 316 breeding varieties(advanced lines).In KJ-RILs,the qPh-3D allele from Jing 411 can significantly reduce PH via reducing all internode lengths without significant effect on spike length(SL),whereas it can reduce kernel yield per plant(KYPP)at certain level.Two markers AX-110160363 and AX-111109273,which closely link with qPh-3D,were used for genotyping in the natural mapping population with the yield-related phenotypic datasets.The qPh-3D allele with decreased PH was found with a positive effect on SL,but a significant negative effect on KYPP.Based on the breeding selection effect analysis at the qPh-3D locus,the qPh-3D allele with decreased PH was present in a higher proportion in wheat varieties released from Beijing and Shanxi in China,but lower in Shandong,Qinghai,Sichuan and the foreign countries.This allele was detected in a lower proportion in earlier varieties,while its presence in modern varieties was detected increasingly.In addition,a closely linked PCR-based InDel marker targeting the qPh-3D locus was developed.Collectively,the findings of this study will provide theoretical guidance for future applications of qPh-3D in molecular breeding programs in wheat.

关 键 词：小麦(Triticum aestivum L.) 株高 qPh-3D 分子标记 遗传效应解析 

分 类 号：S512.1[农业科学—作物学]