分散固相萃取-超高效液相色谱-四极杆/飞行时间质谱法测定禽肉中松香酸类物质  

Determination of abietic acid compounds in poultry by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry coupled with dispersive solid phase extraction

作　　者：林晓君 严婉盈 何敏恒 黄嘉乐 伍依琪 叶玲 张瑞瑞 LIN Xiaojun;YAN Wanying;HE Minheng;HUANG Jiale;WU Yiqi;YE Ling;ZHANG Ruirui(Guangzhou Inspection and Testing Certification Group Co.,Ltd.,National Quality Testing Center for Processed Food〔Guangdong〕,Guangzhou,Guangdong 511447,China)

机构地区：[1]广州检验检测认证集团有限公司国家加工食品质量监督检验中心〔广东〕,广东广州511447

出　　处：《食品与机械》2023年第10期75-79,共5页Food and Machinery

基　　金：广州市市场监督管理局科技项目(编号:2022kj49);广州检验检测认证集团有限公司科技项目(编号:2022kj02GZ)。

摘　　要：目的:建立超高效液相色谱-四极杆/飞行时间质谱法(UPLC-Q-TOF/MS)测定禽肉中松香酸类物质。方法:样品使用乙腈提取,盐析分层后,上清液由C 18固相萃取剂净化,UPLC-Q-TOF/MS电喷雾负离子模式测定。结果:3种松香酸类物质在10~500μg/L质量浓度范围内线性关系良好,相关系数R^(2)>0.998,平均回收率为87.8%~98.5%,相对标准偏差(RSDs)为1.99%~6.71%(n=6);方法检出限(LODs)为10μg/kg,方法定量限(LOQs)为20μg/kg。结论:试验方法操作简便,快捷,回收率和灵敏度高,精密度好,能够满足禽肉中3种松香酸类物质的检测需求。Objective:An ultra performance liquid chromatography-quadrupole/time-of-flight mass spectrometry(UPLC-Q-TOF/MS)method was established for the determination of abietic acids in poultry.Methods:The samples were extracted with acetonitrile,then salted out and stratified.The supernatant was purified by C 18 solid phase extractant and finally determined by UPLC-Q-TOF/MS in ESI negative mode.Results:Abietic acid compounds exhibited a good linear relationship in the concentration range of 10~500μg/L with good correlation coefficient R^(2)>0.998.The average recoveries of three abietic acid compounds were experiments,the average recovery rate was 87.8%~98.5%with relative standard deviations(RSDs,n=6)of 1.99%~6.71%,at the spiked level of 20,40,200μg/kg.The limits of detection(LODs)were 10μg/kg and the method limits of quantification(LOQs)were 20μg/kg.Conclusion:This method was convenient operation,rapid,high recovery and sensitivity and good precision that could meet the determination requirements of three abietic acids in poultry.

关 键 词：分散固相萃取 超高效液相色谱-四极杆/飞行时间质谱 禽肉 松香酸 

分 类 号：O657.63[理学—分析化学] TS251.55[理学—化学]